Govaert Marlies, Smet Cindy, Acquah Cyril, Walsh James L, Van Impe Jan F M
CPMF2 - Flemish Cluster Predictive Microbiology in Foods, Ghent, Belgium.
OPTEC - Optimization in Engineering Center-of-Excellence, KU Leuven, Ghent, Belgium.
Front Microbiol. 2022 Mar 24;13:831434. doi: 10.3389/fmicb.2022.831434. eCollection 2022.
Although the Cold Atmospheric Plasma (CAP) technology proved promising for inactivation of biofilms present on abiotic food contact surfaces, more research is required to examine the behavior of the CAP surviving biofilm-associated cells. It was therefore examined whether (i) CAP treated ( and Typhimurium) biofilm-associated cells were able to further colonize the already established biofilms during a subsequent incubation period and (ii) isolates of the surviving population became less susceptible toward CAP when the number of biofilm development-CAP treatment cycles increased. For this purpose, a direct treatment was applied using a helium-based Dielectric Barrier Discharge electrode configuration. Results indicated that the surviving population was able to further colonize the already established biofilms, since the cell density of the CAP treated + incubated biofilms equaled the initial density of the untreated biofilms. For the biofilms, also the total biomass proved to further increase, which might result in an even further increased resistance. The susceptibility of the biofilm-associated cells proved to be influenced by the specific number of CAP treatment cycles, which might potentially result in an overestimation of the CAP treatment efficacy and, consequently, an increased risk of food contamination.
尽管冷大气等离子体（CAP）技术在灭活非生物食品接触表面上的生物膜方面显示出前景，但仍需要更多研究来考察CAP处理后存活的生物膜相关细胞的行为。因此，研究了以下两个方面：（i）经CAP处理的（鼠伤寒沙门氏菌）生物膜相关细胞在随后的培养期内是否能够进一步定殖于已形成的生物膜中；（ii）当生物膜形成-CAP处理循环次数增加时，存活菌群体的分离株对CAP的敏感性是否降低。为此，采用基于氦的介质阻挡放电电极配置进行直接处理。结果表明，存活菌群体能够进一步定殖于已形成的生物膜中，因为经CAP处理并培养后的生物膜的细胞密度与未处理生物膜的初始密度相当。对于生物膜，总生物量也进一步增加，这可能导致抗性进一步增强。生物膜相关细胞的敏感性受CAP处理循环的特定次数影响，这可能会高估CAP处理效果，从而增加食品污染风险。
