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PgtE 酶与纤溶酶原激活物 (Pla) 在与 DEC-205（CD205）相互作用方面具有相似的生物学作用，并通过 增强宿主的传播和感染力。

PgtE Enzyme of Shares the Similar Biological Roles to Plasminogen Activator (Pla) in Interacting With DEC-205 (CD205), and Enhancing Host Dissemination and Infectivity by .

机构信息

Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan, China.

Department of Pathology, Renmin Hospital of Wuhan University, Wuhan, China.

出版信息

Front Immunol. 2022 Mar 24;13:791799. doi: 10.3389/fimmu.2022.791799. eCollection 2022.

DOI:10.3389/fimmu.2022.791799
PMID:35401532
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8986990/
Abstract

, the cause of plague, is a newly evolved Gram-negative bacterium. Through the acquisition of the plasminogen activator (Pla), gained the means to rapidly disseminate throughout its mammalian hosts. It was suggested that utilizes Pla to interact with the DEC-205 (CD205) receptor on antigen-presenting cells (APCs) to initiate host dissemination and infection. However, the evolutionary origin of Pla has not been fully elucidated. The PgtE enzyme of , involved in host dissemination, shows sequence similarity with the Pla. In this study, we demonstrated that both K-12 and bacteria expressing the PgtE-protein were able to interact with primary alveolar macrophages and DEC-205-transfected CHO cells. The interaction between PgtE-expressing bacteria and DEC-205-expressing transfectants could be inhibited by the application of an anti-DEC-205 antibody. Moreover, PgtE-expressing partially re-gained the ability to promote host dissemination and infection. In conclusion, the DEC-205-PgtE interaction plays a role in promoting the dissemination and infection of , suggesting that Pla and the PgtE of might share a common evolutionary origin.

摘要

鼠疫耶尔森菌是导致鼠疫的病原菌，是一种新近进化的革兰氏阴性菌。通过获得纤溶酶原激活物（Pla），得以迅速在其哺乳动物宿主中传播。有人提出，利用 Pla 与抗原呈递细胞（APCs）上的 DEC-205（CD205）受体相互作用，启动宿主传播和感染。然而，Pla 的进化起源尚未完全阐明。参与宿主传播的 的 PgtE 酶与 Pla 具有序列相似性。在本研究中，我们证明了表达 PgtE 蛋白的 和 K-12 细菌均能够与原代肺泡巨噬细胞和 DEC-205 转染的 CHO 细胞相互作用。用抗 DEC-205 抗体处理可抑制表达 PgtE 的细菌与 DEC-205 表达转染细胞之间的相互作用。此外，表达 PgtE 的 部分恢复了促进宿主传播和感染的能力。总之，DEC-205-PgtE 相互作用在促进 的传播和感染中起作用，表明 Pla 和 的 PgtE 可能具有共同的进化起源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/ac588541e90e/fimmu-13-791799-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/90ea9c5f77de/fimmu-13-791799-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/2fb84a01ff7e/fimmu-13-791799-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/2e9aeebb845a/fimmu-13-791799-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/8e2e4dc25f38/fimmu-13-791799-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/ac588541e90e/fimmu-13-791799-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/90ea9c5f77de/fimmu-13-791799-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/2fb84a01ff7e/fimmu-13-791799-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/2e9aeebb845a/fimmu-13-791799-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/8e2e4dc25f38/fimmu-13-791799-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d75/8986990/ac588541e90e/fimmu-13-791799-g005.jpg

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