Fan Mimi, Pan Zhihui, Wang Chunjuan, Guo Yang, Sun Jingran, Liu Mingzhu, Peng Bo, Wu Jin, Fang Yanjun
The Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environment and Operational Medicine, Tianjin, China.
College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, China.
Front Chem. 2022 Mar 25;10:859379. doi: 10.3389/fchem.2022.859379. eCollection 2022.
A novel ratiometric fluorescence probe based on nitrogen-doped blue carbon dots (NCDs) and red gold nanoclusters (Au NCs) for mercuric ion (Hg2+) has been prepared and characterized. A user friendly fluorescent test paper based sensor combined with smartphone was fabricated for rapid visual and quantitative detection. Hg can specifically bind to Au on the surface of Au NCs, leading to the quench of red fluorescence while the fluorescence intensity of the NCDs with blue fluorescence remained unchanged as a internal standard signal. The implement of paper-based sensor address some common drawback in analytical process such as the detection time, analysis cost. In a further demonstration, a homemade detection device with smartphone was used to qualify the Hg. After adding different concentration of Hg, red, purple, and blue colors were obtained on the detection zones of the fluorescent test paper. The Android App Color Grab was used to identify the red, green and blue (RGB) values of fluorescent color. The rapid visual and quantitative detection of Hg was accomplished with the detection limit of 2.7 nM for fluorescence, 25 nM for smartphone and 32 nM for paper strip. The developed multi-mode detection platform was successfully applied to the detection of mercury ions in water samples with acceptable recoveries. The NCDs and Au NCs probe facilitate the one-site environmental monitoring for Hg with "naked-eye" and smartphone.
制备并表征了一种基于氮掺杂蓝色碳点（NCDs）和红色金纳米簇（Au NCs）的新型汞离子（Hg2+）比率荧光探针。构建了一种结合智能手机的用户友好型荧光试纸传感器，用于快速可视化和定量检测。Hg能特异性结合到Au NCs表面的Au上，导致红色荧光猝灭，而具有蓝色荧光的NCDs的荧光强度作为内标信号保持不变。纸质传感器的应用解决了分析过程中的一些常见缺点，如检测时间、分析成本等。在进一步的演示中，使用带有智能手机的自制检测装置对Hg进行定量。加入不同浓度的Hg后，荧光试纸的检测区域出现红色、紫色和蓝色。利用安卓应用程序Color Grab识别荧光颜色的红、绿、蓝（RGB）值。实现了Hg的快速可视化和定量检测，荧光检测限为2.7 nM，智能手机检测限为25 nM，试纸条检测限为32 nM。所开发的多模式检测平台成功应用于水样中汞离子的检测，回收率良好。NCDs和Au NCs探针有助于通过“肉眼”和智能手机对Hg进行现场环境监测。
