从小鼠多能干细胞再生免疫活性T细胞的两步法方案。
Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells.
作者信息
Wang Tongjie, Lv Cui, Hu Fangxiao, Liu Lijuan, Wang Jinyong
机构信息
State Key Laboratory of Experimental Hematology, CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, 510530, Guangzhou, China.
出版信息
Blood Sci. 2020 Jul 25;2(3):79-88. doi: 10.1097/BS9.0000000000000049. eCollection 2020 Jul.
Numerous efforts have been attempted to regenerate T cells in culture dish from pluripotent stem cells (PSCs). However, in vitro generated T cells exhibited extremely low activity and compromised immunocompetency in vivo. Here, we describe a two-step protocol for regenerating functional T cells using an inducible -PSC (iR9-PSCs) line. The procedure mainly includes generation of induced hematopoietic progenitor cells (iHPCs) in vitro, transplantation, and development of functional induced T cells (iT) in vivo via transplantation. The entire induction process in vitro requires 21 days before iHPCs transplantation. The development of mature T cells in vivo takes 4 to 6 weeks post-transplantation. We provide a simple and reproducible approach for functional T cell regeneration from iR9-PSCs for research purpose.
人们已经进行了许多尝试，试图在培养皿中从多能干细胞（PSC）再生T细胞。然而，体外产生的T细胞在体内表现出极低的活性和受损的免疫能力。在这里，我们描述了一种使用诱导型PSC（iR9-PSC）系再生功能性T细胞的两步方案。该过程主要包括体外诱导造血祖细胞（iHPC）的生成、移植，以及通过移植在体内发育功能性诱导T细胞（iT）。在iHPC移植前，体外的整个诱导过程需要21天。移植后，体内成熟T细胞的发育需要4至6周。我们提供了一种简单且可重复的方法，用于从iR9-PSC再生功能性T细胞，以用于研究目的。
Zotero 插件