Beijing Engineering Research Center of Protein and Functional Peptides, China National Research Institute of Food and Fermentation Industries, Beijing, People's Republic of China.
Engineering Laboratory for Agro Biomass Recycling & Valorizing, College of Engineering, China Agricultural University, Beijing, People's Republic of China.
J Food Biochem. 2022 Aug;46(8):e14162. doi: 10.1111/jfbc.14162. Epub 2022 Apr 11.
In this study, the effect of corn oligopeptides (COPs) with liver protection activity on mice with hepatic fibrosis (HF) induced by carbon tetrachloride (CCl ) was studied. It was proved that COPs can ameliorate the liver injury and inflammation caused by CCl by histopathology and enzyme-linked immunosorbent assay in mice. The expression of Akt/NF-κB inflammatory pathway was determined by real-time polymerase chain reaction (RT-PCR) and western blotting (WB). The results showed that COPs inhibited the expression of key proteins in the inflammatory pathway. In conclusion, the results of this study suggested that COPs could improve CCl -induced HF by improving liver injury, reducing the expression of inflammatory factors, and inhibiting the expression of inflammatory signaling pathways. PRACTICAL APPLICATIONS: The corns around the world are mainly used as animal feed, and the liver protective activity of corn oligopeptides (COPs) is rarely applied to the market. The development of COPs liver protective food can prevent the occurrence of liver-related diseases such as hepatic fibrosis to a certain extent. Developing COPs liver protecting food can improve the utilization value of corn. It is hoped that this study can provide experimental support for the application of COPs in liver protection food.
本研究探讨了具有保肝活性的玉米低聚肽（COPs）对四氯化碳（CCl ）诱导的肝纤维化（HF）小鼠的作用。通过组织病理学和酶联免疫吸附试验（ELISA）证明，COPs 可改善 CCl 诱导的小鼠肝损伤和炎症。通过实时聚合酶链反应（RT-PCR）和蛋白质印迹法（WB）测定 Akt/NF-κB 炎症通路的表达。结果表明，COPs 抑制了炎症通路中关键蛋白的表达。总之，本研究结果表明，COPs 可通过改善肝损伤、降低炎症因子的表达和抑制炎症信号通路的表达，改善 CCl 诱导的 HF。实际应用：全世界的玉米主要用作动物饲料，玉米低聚肽（COPs）的保肝活性很少应用于市场。开发 COPs 保肝食品可以在一定程度上预防与肝脏相关的疾病，如肝纤维化。开发 COPs 保肝食品可以提高玉米的利用价值。希望本研究能为 COPs 在保肝食品中的应用提供实验支持。
