眼移植物抗宿主病患者的泪膜免疫特征。
Tear Film Immunological Profile in Patients with Ocular Graft versus Host Disease.
机构信息
Department of Ophthalmology and Visual Sciences, Federal University of São Paulo, São Paulo, Brazil.
Department of Immunology, Institute of Biomedical Sciences IV, University of São Paulo, São Paulo, Brazil.
出版信息
Ocul Immunol Inflamm. 2023 May;31(4):701-709. doi: 10.1080/09273948.2022.2046794. Epub 2022 Apr 11.
PURPOSE
To analyze and compare the tear immunological profile in ocular GVHD (oGVHD) patients with that in non-oGVHD patients and to correlate them with ocular surface parameters based on the International Chronic Ocular GVHD Consensus Group (ICCGVHD) diagnostic criteria.
METHODS
Tear samples from 20 individuals who underwent allo-hematopoietic stem cell transplantation and were grouped according the presence or absence of oGVHD were analyzed using Bio-Plex assay.
RESULTS
IL-8 and MIP-1α levels were significantly higher in tears from oGVHD patients compared with those in tears from non-oGVHD patients (p<0.001 and p=0.001, respectively). Tear IL-8 levels correlated significantly with OSDI criteria (ρ=0.5159, p=0.001), ocular hyperemia (ρ=0.469, p=0.002), and corneal staining (ρ=0.339, p=0.032), whereas tear Mip-1α levels correlated with OSDI score (ρ=0.358, p=0.023).
CONCLUSION
We demonstrated higher tear levels of IL-8 and MIP-1α in oGVHD patients and significant correlations between theses cytokines and ocular surface parameters based on the ICCGVHDCG criteria.
目的
根据国际慢性移植物抗宿主病共识组（ICCGVHD）的诊断标准，分析和比较眼移植物抗宿主病（oGVHD）患者与非 oGVHD 患者的泪液免疫谱，并将其与眼表参数相关联。
方法
使用 Bio-Plex 检测法分析了 20 名接受异基因造血干细胞移植并根据是否存在 oGVHD 分组的个体的泪液样本。
结果
与非 oGVHD 患者相比，oGVHD 患者的泪液中 IL-8 和 MIP-1α 水平明显更高（p<0.001 和 p=0.001）。泪液 IL-8 水平与 OSDI 标准显著相关（ρ=0.5159，p=0.001），与眼充血（ρ=0.469，p=0.002）和角膜染色（ρ=0.339，p=0.032）也显著相关，而泪液 MIP-1α 水平与 OSDI 评分相关（ρ=0.358，p=0.023）。
结论
我们证明了 oGVHD 患者的泪液中 IL-8 和 MIP-1α 水平更高，并且这些细胞因子与基于 ICCGVHDCG 标准的眼表参数之间存在显著相关性。
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