Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, Australia; Faculty of Nursing, Federal University of Goias, Goiania, Brazil.
Macquarie University Hospital, Macquarie University, Sydney, Australia.
J Hosp Infect. 2022 Jun;124:67-71. doi: 10.1016/j.jhin.2022.03.011. Epub 2022 Apr 9.
The efficacy of double manual cleaning (DMC) with enzymatic detergent followed by alkaline detergent on biofilm removal on hinged surgical instruments was compared to automated cleaning. Biofilm-covered haemostatic forceps were divided into four groups: positive control (soaked in sterile water); DMC; DMC plus extra brushing of the inner hinge; and automated cleaning. All DMC, DMC plus brushing the hinge, and automated cleaning significantly (P < 0.001) reduced 94.8%, 99.8%, and 100% viable bacteria and 82.3%, 93.8%, and 95.1% residual protein, respectively, compared to positive control. DMC instruments had significantly more viable bacteria (P < 0.05) and residual protein (P < 0.01) than those in instruments subjected to DMC with hinge brushing and automated cleaning. However, there was no significant difference in residual protein between DMC with hinge brushing and automated cleaning. In sterilizing service units with no access to automated cleaning equipment, it is important to brush the inner hinge during manual cleaning, and DMC plus brushing the inner hinge could be considered a viable alternative for cleaning hinged surgical instruments.
对比手动清洗（DMC）加酶清洁剂和碱性清洁剂、全自动清洗对铰链手术器械生物膜的去除效果。将附有生物膜的止血夹分成四组：阳性对照组（浸泡在无菌水中）；DMC 组；DMC 加内铰链额外刷洗组；全自动清洗组。与阳性对照组相比，DMC、DMC 加铰链刷洗和全自动清洗组均能显著减少 94.8%、99.8%和 100%的活菌数（P < 0.001），分别减少 82.3%、93.8%和 95.1%的残留蛋白（P < 0.001）。DMC 组的活菌数（P < 0.05）和残留蛋白（P < 0.01）明显高于铰链刷洗和全自动清洗组。然而，铰链刷洗和全自动清洗组之间的残留蛋白无显著差异。在没有全自动清洗设备的消毒服务单位，手动清洗时对内铰链进行刷洗非常重要，DMC 加铰链刷洗可以作为铰链手术器械清洗的一种可行替代方法。
