Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China.
Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China.
Bioelectrochemistry. 2022 Aug;146:108131. doi: 10.1016/j.bioelechem.2022.108131. Epub 2022 Apr 6.
In electrochemical immunoassays, great breakthroughs have been made in ultrasensitive detection of tumor markers by amplifying signals with coreaction accelerators. Herein, carbon nitrides with nitrogen vacancies (CNNVs) are proposed as emitter, due to the introduction of nitrogen vacancies this emitter has better ECL efficiency, the phenomena of interface electron transfer and electrode passivation are improved. At the same time, it can also promote the electrochemical reduction of coreactant, making it an attractive and potential emitter. The electrode was modified with Ag-TiC MXene. It not only accelerates electron transfer and increases the effective working area of the electrode, but also acts as a coreaction accelerator to promote the electrochemical reduction of the coreactant KSO, resulting in a strong ECL signal. The immunosensor showed a good linear relationship in the range of 10-100 ng/mL with a detection limit of 0.36 fg/mL. In addition, the excellent properties of good specificity and ultra-high stability provide an effective method for ultra-sensitive immunoassays.
在电化学生物免疫分析中，通过使用共反应加速剂来放大信号，在超灵敏检测肿瘤标志物方面取得了重大突破。在此，提出具有氮空位的碳氮化物（CNNVs）作为发射器，由于引入了氮空位，这种发射器具有更好的 ECL 效率，改善了界面电子转移和电极钝化现象。同时，它还可以促进共反应物的电化学还原，使其成为一种有吸引力和潜在的发射器。电极用 Ag-TiC MXene 进行了修饰。它不仅加速了电子转移并增加了电极的有效工作面积，而且还充当共反应加速剂来促进共反应物 KSO 的电化学还原，从而产生强的 ECL 信号。该免疫传感器在 10-100ng/mL 的范围内呈现出良好的线性关系，检测限为 0.36fg/mL。此外，良好的特异性和超高稳定性的优异性能为超灵敏免疫分析提供了一种有效的方法。
