Liu Junqing, Wang Hao, Xu Dongxin, Li Yan, Fang Jiaru, Zhang Mingyue, Xia Qijian, Huang Zhanyun, Cao Nan, Hu Ning, Guo Jun
Department of Cardiology, The First Affiliated Hospital of Jinan University, Guangzhou, 510630, China.
State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-Sen University, Guangzhou, 510006, China.
Biosens Bioelectron. 2022 Aug 1;209:114252. doi: 10.1016/j.bios.2022.114252. Epub 2022 Apr 6.
In cardiac tissue engineering, electric stimulation is an efficient approach to improve the formation of cardiac tissue from individual cardiomyocyte. The regulation conditions of electric stimulation should be screened in an efficient way. However, the lack of high-throughput and large-scale assessment platforms limited the effectively screen the regulation conditions. Here, we develop a high-throughput integrated electrical stimulation system to rhythmically regulate the cardiomyocytes in situ. The state of regulated cardiomyocytes is characterized by a video-based automated biosensing system to analyze the beating of cardiomyocytes. Electrical stimulation conditions are optimized to regulate the cardiomyocyte state in vitro to replace the complex bioactive molecules and materials. By the video analysis, the accurate beating rate and regularity of cardiomyocyte can be determined. The results show that electrical stimulation frequency is a significant factor to regulate the cardiomyocyte beating. The electrical stimulation with a frequency of 3 Hz can effectively regulate the primary rat cardiomyocytes with normal rhythm. This high-throughput electrical stimulation and a video-based automated biosensing system will be a promising and powerful tool to effectively optimize the regulation conditions of cardiomyocyte in vitro, and possess broad application prospects in cardiac tissue engineering and pharmaceutical industry.
在心脏组织工程中，电刺激是一种促进单个心肌细胞形成心脏组织的有效方法。电刺激的调控条件需要以高效的方式进行筛选。然而，缺乏高通量和大规模评估平台限制了对调控条件的有效筛选。在此，我们开发了一种高通量集成电刺激系统，用于原位有节律地调控心肌细胞。通过基于视频的自动生物传感系统对受调控心肌细胞的状态进行表征，以分析心肌细胞的搏动。优化电刺激条件以在体外调控心肌细胞状态，从而取代复杂的生物活性分子和材料。通过视频分析，可以确定心肌细胞准确的搏动频率和规律性。结果表明，电刺激频率是调节心肌细胞搏动的一个重要因素。频率为3Hz的电刺激能够有效地以正常节律调控原代大鼠心肌细胞。这种高通量电刺激和基于视频的自动生物传感系统将成为有效优化体外心肌细胞调控条件的一种有前景且强大的工具，在心脏组织工程和制药行业具有广阔的应用前景。
