Sutherland Donna L, Bramucci Anna
Faculty of Science, University of Technology Sydney, Ultimo NSW, 2007, Australia.
Faculty of Science, University of Technology Sydney, Ultimo NSW, 2007, Australia.
J Environ Manage. 2022 Jul 1;313:115018. doi: 10.1016/j.jenvman.2022.115018. Epub 2022 Apr 8.
Dissolved organic phosphorus (DOP) accounts for a substantial proportion of the total phosphorus remaining in the wastewater discharge and remains a concern for the receiving environment. This study assessed the potential of wastewater microalgae for the bioremediation of DOP from anaerobically digested food-waste centrate. For high DOP to low DIP ratio, the microalgal consortia was able to remove over 98% of DOP and 95% of total dissolved phosphorus. However, under a 1:1 ratio of DOP to DIP, the microalgal consortia was only able to remove 5% of the organic phosphorus and 76% of total dissolved phosphorus. All five main microalgal species were capable of producing alkaline phosphatase to some degree, the enzyme responsible for hydrolysing the phosphorus. For the dominant species Desmodesmus communis, total phosphatase activity reduced from 46.0 ± 2.3 mmol L h in axenic cultures to only 6.3 ± 0.7 mmol L h in presence of its microbiome. This resulted in a reduction in biomass from 209 ± 13 g m to 73 ± 5 g m. For Tetradesmus dimorphus, alkaline phosphatase increased from 6.5 ± 0.3 mmol L h in the axenic culture to 169.8 ± 40.1 mmol L h in presence of both its microbiome and centrate-sourced bacteria but had little impact on biomass production. DOP removal rates across all five species, in all treatments ranged from 17 to 91%. With the exception of D. communis, the nutrient removal efficiency of DOP per unit biomass suggested luxury uptake of phosphorus into the microalgal cell. For wastewaters with low inorganic and moderate to high organic phosphorus microalgal-based wastewater treatment systems may offer a cost-effective mechanism for the removal and recovery of dissolved organic phosphorus from wastewater. Further research on refining organic phosphorus bioremediation in a range of wastewater types, particularly at pilot and full-scale, is needed.
溶解有机磷（DOP）在废水排放中剩余的总磷中占相当大的比例，仍然是受纳环境关注的问题。本研究评估了废水微藻对厌氧消化食物垃圾浓缩液中DOP进行生物修复的潜力。对于高DOP与低溶解性无机磷（DIP）比例的情况，微藻群落能够去除超过98%的DOP和95%的总溶解磷。然而，在DOP与DIP比例为1:1的情况下，微藻群落仅能去除5%的有机磷和76%的总溶解磷。所有五种主要微藻物种都能在一定程度上产生碱性磷酸酶，该酶负责水解磷。对于优势物种普通栅藻，总磷酸酶活性从无菌培养中的46.0±2.3 mmol L⁻¹ h⁻¹降至在其微生物群落存在时的仅6.3±0.7 mmol L⁻¹ h⁻¹。这导致生物量从209±13 g m⁻²降至73±5 g m⁻²。对于双形四棘藻，碱性磷酸酶在无菌培养中为6.5±0.3 mmol L⁻¹ h⁻¹，在其微生物群落和浓缩液来源细菌都存在时增加到169.8±40.1 mmol L⁻¹ h⁻¹，但对生物量生产影响不大。在所有处理中，所有五个物种的DOP去除率在17%至91%之间。除普通栅藻外，单位生物量对DOP的营养物去除效率表明磷被微藻细胞大量吸收。对于无机磷含量低且有机磷含量中等至高的废水，基于微藻的废水处理系统可能为从废水中去除和回收溶解有机磷提供一种具有成本效益的机制。需要进一步研究在一系列废水类型中优化有机磷生物修复，特别是在中试和全规模水平。
