使用外显子特异性cDNA探针进行HLA - DR同种基因分型及快速微型凝胶方法的应用

HLA-DR allogenotyping using exon-specific cDNA probes and application of rapid minigel methods.

作者信息

Bidwell J L, Jarrold E A

出版信息

Mol Immunol. 1986 Oct;23(10):1111-6. doi: 10.1016/0161-5890(86)90009-x.

Abstract

The construction of exon-specific HLA-DR beta cDNA clones and their application as DNA or RNA hybridization probes in DR allogenotyping are described. Using a probe derived from the beta 2, transmembrane, cytoplasmic and 3'-untranslated exons of the DR beta chain gene (pRTV1), it is possible to discriminate between DR alleles using the restriction endonucleases TaqI or BamHI. Micro-scale chromosomal DNA isolation procedures, used in conjunction with Southern blot analysis in minigels, facilitate the rapid identification of DR allogenotypes without significant loss of hybridization signal resolution.

摘要

本文描述了外显子特异性HLA - DRβ cDNA克隆的构建及其作为DNA或RNA杂交探针在DR同种异体基因分型中的应用。使用源自DRβ链基因的β2、跨膜、细胞质和3' - 非翻译外显子的探针（pRTV1），通过限制性内切酶TaqI或BamHI可以区分DR等位基因。与微型凝胶中的Southern印迹分析相结合使用的微量染色体DNA分离程序，有助于快速鉴定DR同种异体基因型，而不会显著损失杂交信号分辨率。