Lee Solji, Jeon Donghyun, Choi Sehyun, Kang Yuna, Seo Sumin, Kwon Soonjae, Lyu Jaeil, Ahn Joonwoo, Seo Jisu, Kim Changsoo
Department of Crop Science, Chungnam National University, Daejeon 34134, Korea.
Department of Smart Agriculture Systems, Chungnam National University, Daejeon 34134, Korea.
Plants (Basel). 2022 Mar 24;11(7):869. doi: 10.3390/plants11070869.
Salinity stress is one of the most important abiotic stresses that causes great losses in crop production worldwide. Identifying the molecular mechanisms of salt resistance in sorghum will help develop salt-tolerant crops with high yields. Sorghum ( (L.) Moench) is one of the world's four major grains and is known as a plant with excellent adaptability to salt stress. Among the various genotypes of sorghum, a Korean cultivar Nampungchal is also highly tolerant to salt. However, little is known about how Nampungchal responds to salt stress. In this study, we measured various physiological parameters, including Na and K contents, in leaves grown under saline conditions and investigated the expression patterns of differentially expressed genes (DEGs) using QuantSeq analysis. These DEG analyses revealed that genes up-regulated in a 150 mM NaCl treatment have various functions related to abiotic stresses, such as ERF and DREB. In addition, transcription factors such as ABA, WRKY, MYB, and bZip bind to the CREs region of sorghum and are involved in the regulation of various abiotic stress-responsive transcriptions, including salt stress. These findings may deepen our understanding of the mechanisms of salt tolerance in sorghum and other crops.
盐胁迫是最重要的非生物胁迫之一,在全球范围内导致作物产量大幅损失。确定高粱耐盐性的分子机制将有助于培育高产耐盐作物。高粱((L.) Moench)是世界四大谷物之一,以对盐胁迫具有出色适应性的植物而闻名。在高粱的各种基因型中,韩国品种南丰chal也对盐具有高度耐受性。然而,关于南丰chal如何响应盐胁迫知之甚少。在本研究中,我们测量了在盐胁迫条件下生长的叶片中的各种生理参数,包括钠和钾含量,并使用QuantSeq分析研究了差异表达基因(DEG)的表达模式。这些DEG分析表明,在150 mM NaCl处理中上调的基因具有与非生物胁迫相关的各种功能,如ERF和DREB。此外,ABA、WRKY、MYB和bZip等转录因子与高粱的CREs区域结合,并参与包括盐胁迫在内的各种非生物胁迫响应转录的调控。这些发现可能会加深我们对高粱和其他作物耐盐机制的理解。