Sharma Himanshu, Sharma Alok, Rajput Ruchika, Sidhu Sukhjeet, Dhillon Harpal, Verma Praveen Chandra, Pandey Ashutosh, Upadhyay Santosh Kumar
Department of Botany, Panjab University, Chandigarh 160014, India.
Department of Bio-Technology, I.K. Gujral Punjab Technical University, Kapurthala 144603, India.
Plants (Basel). 2022 Mar 29;11(7):911. doi: 10.3390/plants11070911.
Boron (B) is an essential micronutrient of plants. Plants grapple with a narrow range of B between its toxicity and deficiency. B homeostasis mechanism is required to rescue plants from such a quagmire. B transporters are specialized proteins involved in the homeostasis of B. In the present study, a total of 29 genes were identified in five major cereals, including three in each and , four in , six in , and 13 in . Multiple sequence alignments, domain structure analyses, and phylogenetic analysis indicated the conserved nature of the BOR protein family. Duplication events and Ka/Ks analysis of showed the role of segmental duplication events and purifying selection in the expansion of the BOR family in . Furthermore, in silico expression and co-expression analyses under biotic and abiotic stress conditions depicted their involvement in combating such conditions. Moreover, qRT-PCR of in B treatment suggested the roles of genes in B stress management. The present study hints at the conserved nature of BOR proteins and their different aspects. The study will lay down a way for several crop improvement programs.
硼（B）是植物必需的微量营养元素。植物在硼的毒性和缺乏之间面临着狭窄的硼浓度范围。需要硼稳态机制来使植物摆脱这种困境。硼转运蛋白是参与硼稳态的特殊蛋白质。在本研究中，在五种主要谷物中总共鉴定出29个基因，其中每种谷物中分别有3个、4个、6个和13个。多序列比对、结构域结构分析和系统发育分析表明了BOR蛋白家族的保守性质。对[具体谷物名称未给出]的复制事件和Ka/Ks分析表明了片段重复事件和纯化选择在[具体谷物名称未给出]中BOR家族扩展中的作用。此外，在生物和非生物胁迫条件下的电子表达和共表达分析表明它们参与应对这些条件。此外，在硼处理下对[具体谷物名称未给出]进行qRT-PCR表明[具体谷物名称未给出]基因在硼胁迫管理中的作用。本研究揭示了BOR蛋白的保守性质及其不同方面。该研究将为多个作物改良计划奠定基础。
