Tang Xue-Mei, Liu Pan-Dao, Chen Zhi-Jian, Li Xin-Yong, Huang Rui, Liu Guo-Dao, Dong Rong-Shu, Chen Jian
Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, China.
Key Laboratory of Food Nutrition and Functional Food of Hainan Province, One Health Institute, Engineering Research Center of Utilization of Tropical Polysaccharide Resources, Ministry of Education, College of Food Science and Technology, Hainan University, Haikou 570228, China.
Foods. 2022 Mar 24;11(7):936. doi: 10.3390/foods11070936.
Owing to their excellent characteristics, Pickering emulsions have been widely used in the development and the application of new carriers for embedding and for delivering active compounds. In this study, β-carotene was successfully encapsulated in a Pickering emulsion stabilized using protein isolate (DIPI). The results showed that the encapsulation efficiencies of β-carotene in the control group Tween 20 emulsion (TE) and the DIPI Pickering emulsion (DIPIPE) were 46.7 ± 2.5% and 97.3 ± 0.8%, respectively. After storage for 30 days at 25 °C and 37 °C in a dark environment, approximately 79.4% and 72.1% of β-carotene in DIPIPE were retained. Compared with TE, DIPIPE can improve the stability of β-carotene during storage. In vitro digestion experiments showed that the bioaccessibility rate of β-carotene in DIPIPE was less than that in TE. Cytotoxicity experiments showed that DIPI and β-carotene micelles within a specific concentration range exerted no toxic effects on 3T3 cells. These results indicate that DIPIPE can be used as a good food-grade carrier for embedding and transporting active substances to broaden the application of the protein-based Pickering emulsion system in the development of functional foods.
由于具有优异的特性，皮克林乳液已广泛应用于新型载体的开发以及活性化合物的包埋和递送应用中。在本研究中，β-胡萝卜素成功地被包封在由分离蛋白（DIPI）稳定的皮克林乳液中。结果表明，β-胡萝卜素在对照组吐温20乳液（TE）和DIPI皮克林乳液（DIPIPE）中的包封率分别为46.7±2.5%和97.3±0.8%。在25℃和37℃黑暗环境中储存30天后，DIPIPE中约79.4%和72.1%的β-胡萝卜素得以保留。与TE相比，DIPIPE可提高β-胡萝卜素在储存期间的稳定性。体外消化实验表明，DIPIPE中β-胡萝卜素的生物可及率低于TE。细胞毒性实验表明，特定浓度范围内的DIPI和β-胡萝卜素胶束对3T3细胞无毒性作用。这些结果表明，DIPIPE可作为一种良好的食品级载体用于包埋和运输活性物质，以拓宽基于蛋白质的皮克林乳液体系在功能性食品开发中的应用。
