Park Se-Jun, Shon Dong-Hyun, Ryu Yang-Hwan, Ko Yong
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, Korea.
NMS Labs, 311, Anyang-ro, Manan-gu, Anyang-si 14001, Korea.
Foods. 2022 Apr 1;11(7):1028. doi: 10.3390/foods11071028.
Browning of adipocytes using herbal extracts is an attractive and realistic strategy for obesity treatment. () is an Asian traditional medicine known to activate brown adipocytes. To evaluate the effect of (EEs) on the browning of white adipocytes, expression levels of browning markers, including uncoupling protein 1 (UCP1), were determined using qPCR, Western blot, and immunocytochemistry after mature mouse inguinal preadipocyte (mIPA) and human adipose-derived stem cells (hADSCs) were treated with EEs. In addition, mitochondrial activity was determined by analyzing MitoTracker staining, mtDNA copy number, and oxygen consumption rate (OCR). Treatment with EEs suppressed lipid accumulation and expression levels of adipogenic markers, including , during mIPA differentiation. In mature mIPA and hADSCs browning markers, including , were up-regulated by EEs. In addition, EEs increased expression of mitochondrial genes, mtDNA copy number, and OCR. EEs showed a dual function: inhibiting adipogenesis in immature preadipocytes, and promoting thermogenesis via browning in mature white adipocytes. Therefore, is a potential herb for regulating energy metabolism by inducing the browning process.
使用草药提取物使脂肪细胞褐变是一种有吸引力且切实可行的肥胖治疗策略。（）是一种已知可激活褐色脂肪细胞的亚洲传统药物。为了评估（提取物）对白色脂肪细胞褐变的影响，在成熟小鼠腹股沟前脂肪细胞（mIPA）和人脂肪来源干细胞（hADSCs）用提取物处理后，使用qPCR、蛋白质免疫印迹和免疫细胞化学法测定包括解偶联蛋白1（UCP1）在内的褐变标志物的表达水平。此外，通过分析MitoTracker染色、线粒体DNA拷贝数和氧消耗率（OCR）来测定线粒体活性。在mIPA分化过程中，提取物处理可抑制脂质积累和包括（此处原文缺失具体物质）在内的脂肪生成标志物的表达水平。在成熟的mIPA和hADSCs中，包括（此处原文缺失具体物质）在内的褐变标志物被提取物上调。此外，提取物增加了线粒体基因的表达、线粒体DNA拷贝数和OCR。提取物具有双重功能：抑制未成熟前脂肪细胞的脂肪生成，并通过成熟白色脂肪细胞的褐变促进产热。因此，（此处原文缺失具体物质）是一种通过诱导褐变过程来调节能量代谢的潜在草药。
