Trummer Florian, Glatter Otto, Chemelli Angela
Institute of Inorganic Chemistry, Faculty of Technical Chemistry, Chemical and Process Engineering, Biotechnology, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria.
Nanomaterials (Basel). 2022 Mar 29;12(7):1133. doi: 10.3390/nano12071133.
In contrast to their more common counterparts in aqueous solutions, inverse ISAsomes (internally self-assembled somes/particles) are formulated as kinetically stabilised dispersions of hydrophilic, lyotropic liquid-crystalline (LC) phases in non-polar oils. This contribution reports on their formation in bio-compatible oils. We found that it is possible to create inverse hexosomes, inverse micellar cubosomes (Fd3m) and an inverse emulsified microemulsion (EME) in excess squalane with a polyethylene glycol alkyl ether as the primary surfactant forming the LC phase and to stabilise them with hydrophobised silica nanoparticles. Furthermore, an emulsified L1-phase and inverse hexosomes were formed in excess triolein with the triblock-copolymer Pluronic P94 as the primary surfactant. Stabilisation was achieved with a molecular stabiliser of type polyethylene glycol (PEG)-dipolyhydroxystearate. For the inverse hexosomes in triolein, the possibility of a formulation without any additional stabiliser was explored. It was found that a sufficiently strong stabilisation effect was created by the primary surfactant alone. Finally, triolein was replaced with olive oil which also led to the successful formation of inverse hexosomes. As far as we know, there exists no previous contribution about inverse ISAsomes in complex oils such as triolein or plant oils, and the existence of stabiliser-free (i.e., self-stabilising) inverse hexosomes has also not been reported until now.
与水溶液中更为常见的对应物不同，反相ISAsomes（内部自组装体/颗粒）被制备为亲水性溶致液晶（LC）相在非极性油中的动力学稳定分散体。本文报道了它们在生物相容性油中的形成情况。我们发现，以聚乙二醇烷基醚作为形成LC相的主要表面活性剂，并使用疏水化二氧化硅纳米颗粒对其进行稳定化处理，可以在过量角鲨烷中制备反相六方液晶囊泡、反相胶束立方液晶（Fd3m）和反相乳化微乳液（EME）。此外，以三嵌段共聚物普朗尼克P94作为主要表面活性剂，在过量三油酸甘油酯中形成了乳化L1相和反相六方液晶囊泡。使用聚乙二醇（PEG）-二聚羟基硬脂酸酯类型的分子稳定剂实现了稳定化。对于三油酸甘油酯中的反相六方液晶囊泡，探索了不添加任何额外稳定剂的配方可能性。结果发现，仅主要表面活性剂就能产生足够强的稳定化效果。最后，用橄榄油替代三油酸甘油酯，也成功形成了反相六方液晶囊泡。据我们所知，此前尚无关于在三油酸甘油酯或植物油等复杂油中反相ISAsomes的报道，而且迄今为止也未报道过无稳定剂（即自稳定）反相六方液晶囊泡的存在。
