Henan Provincial Coal Geological Survey and Research Institute, Zhengzhou 450052, China.
Key Laboratory of Water and Soil Resources Protection and Rehabilitation in the Middle and Lower Reaches of the Yellow River Basin, Ministry of Natural Resources, Zhengzhou 450052, China.
Molecules. 2022 Mar 24;27(7):2092. doi: 10.3390/molecules27072092.
In this study, a rapid and simple method based on accelerated solvent extraction (ASE) combined with gas chromatography-mass spectrometry (GC-MS) was established to determine the levels of aniline in soil. The matrix spike recovery rates of aniline were investigated by changing several experimental parameters such as vacuum freeze-drying, accelerated solvent extraction, sample transfer, nitrogen-blowing concentration and solvent exchange. Under optimized pretreatment conditions, the linearity of the method ranged from 0.5 to 20 μg mL for aniline, and the correlation coefficient was 0.999. Recoveries of aniline from quartz sand and soil ranged from 76% to 98%, while the precision was excellent with average inter-day and intraday values ranging (n = 6) from 3.1% to 7.5% and 2.0% to 6.9%, respectively. The limits of quantification of the method were 0.04 mg kg. Notably, the results show that the method we developed is simple, fast, low cost and can meet the requirements for the determination of aniline in soil samples, sewage sludge, river and pond sediments.
本研究建立了一种基于加速溶剂萃取（ASE）结合气相色谱-质谱（GC-MS）的快速简便方法，用于测定土壤中苯胺的含量。通过改变真空冷冻干燥、加速溶剂萃取、样品转移、氮吹浓缩和溶剂交换等几个实验参数，考察了苯胺的基质加标回收率。在优化的预处理条件下，该方法对苯胺的线性范围为 0.5-20μg mL，相关系数为 0.999。从石英砂和土壤中回收苯胺的回收率为 76%-98%，而精密度良好，日间和日内平均回收率分别为 3.1%-7.5%和 2.0%-6.9%（n=6）。该方法的定量限为 0.04mg kg。值得注意的是，结果表明，我们开发的方法简单、快速、低成本，能够满足土壤样品、污水污泥、河流和池塘沉积物中苯胺测定的要求。
