Food Analysis and Research Laboratory, Bingöl University, Bingöl 12000, Turkey.
Department of Chemistry, Bingöl University, Bingöl 12000, Turkey.
Molecules. 2022 Apr 1;27(7):2306. doi: 10.3390/molecules27072306.
A new feature of hydrophobic fumed silica nanoparticles (HFSNPs) when they apply to the preparation of monolithic nano-columns using narrow monolithic fused silica capillary columns (e.g., 50-µm inner diameter) was presented. The monolithic nano-columns were synthesized by an in-situ polymerization using butyl methacrylate (BMA) and ethylene dimethacrylate (EDMA) at various concentrations of AEROSILR972, called HFSNPs. Dimethyl formamide (DMF) and water were used as the porogenic solvents. These columns (referred to as HFSNP monoliths) were successfully characterized by using scanning electron microscopy (SEM) and reversed-phase nano-LC using alkylbenzenes and polyaromatic hydrocarbons as solute probes. The reproducibility values based on run-to-run, column-to-column and batch-to-batch were found as 2.3%, 2.48% and 2.99% ( = 3), respectively. The optimized column also indicated promising hydrophobic interactions under reversed-phase conditions, while the feasibility of the column allowed high efficiency and high throughput nano-LC separations. The potential of the final HFSNP monolith in relation to intact protein separation was successfully demonstrated using six intact proteins, including ribonuclease A, cytochrome C, carbonic anhydrase isozyme II, lysozyme, myoglobin, and α-chymotrypsinogen A in nano-LC. The results showed that HFSNP-based monolithic nanocolumns are promising materials and are powerful tools for sensitive separations.
当疏水型气相法二氧化硅纳米颗粒 (HFSNPs) 应用于使用窄径整体熔融石英毛细管柱（例如 50-µm 内径）制备整体纳米柱时，表现出一个新的特性。通过在原位聚合反应中使用甲基丙烯酸丁酯 (BMA) 和二乙二醇二甲基丙烯酸酯 (EDMA) ，并以不同浓度的 AEROSILR972（称为 HFSNPs）作为致孔剂溶剂，合成了整体纳米柱。这些柱（称为 HFSNP 整体柱）成功地通过扫描电子显微镜 (SEM) 和反相纳流 LC 进行了表征，使用烷基苯和多环芳烃作为溶质探针。基于运行到运行、柱到柱和批到批的重现性值分别为 2.3%、2.48%和 2.99%（n = 3）。优化后的柱在反相条件下也表现出良好的疏水相互作用，同时该柱的可行性允许高效和高通量的纳流 LC 分离。使用六组完整蛋白质，包括核糖核酸酶 A、细胞色素 C、碳酸酐酶同工酶 II、溶菌酶、肌红蛋白和α-糜蛋白酶原 A，成功地在纳流 LC 中证明了最终 HFSNP 整体柱在完整蛋白质分离方面的潜力。结果表明，基于 HFSNP 的整体纳米柱是有前途的材料，是用于灵敏分离的有力工具。
