Department of Horticulture, Division of Applied Life Science (BK21 Four), Graduate School of Gyeongsang National University, Jinju 52828, Korea.
Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea.
Int J Mol Sci. 2022 Mar 30;23(7):3803. doi: 10.3390/ijms23073803.
Silicon (Si) has beneficial effects on not only plant growth but also against biotic and abiotic stresses. However, a few studies focus on how Si application helps strawberry ( × Duch.) resist powdery mildew. The aim of this work was to find out the optimal Si application method before cutting propagation to enhance the resistance to powdery mildew in strawberry "daughter" plants. Naturally infected "mother" plants of 'Sulhyang', 'Maehyang', and 'Kuemsil' strawberries were supplied with Si. Potassium silicate (KSiO) at a final concentration of 75 mg·L Si was either added to the medium for drenching or sprayed to the leaves of the "mother" or "daughter" plant, or soluble Si fertilizer was used to dress the "mother" plant. The Si application significantly increased the shoot fresh weight of the "daughter" plants. Supplemental Si also increased the contents of phosphorus (P), potassium (K), and magnesium (Mg). In addition, the Si treatment decreased the damage of powdery mildew by increased level of proline content and suppressive reactive oxygen species. After applying Si, the length and density of hyphae on the leaf surface decreased. In addition, the infected area of "daughter" plant leaves covered with powdery mildew decreased. This study also demonstrated that Si increased the expression of resistance-gene and decreased the expression of susceptibility-gene of strawberry. Overall, Si application promoted the growth of the "daughter" plants regardless of the application method. Direct foliar Si spray to the "daughter" plants before cutting propagation is recommended to increase their resistance to powdery mildew.
硅（Si）不仅对植物生长有有益影响，而且对生物和非生物胁迫也有抗性。然而，只有少数研究关注硅的应用如何帮助草莓（ × Duch.）抵抗白粉病。本工作旨在确定在切割繁殖前施加硅的最佳方法，以增强草莓“女儿”植株对白粉病的抗性。自然感染的“母亲”植株供应硅。最终浓度为 75 mg·L Si 的硅酸钾（KSiO）要么添加到浸种的培养基中，要么喷洒到“母亲”或“女儿”植株的叶子上，要么使用可溶性硅肥给“母亲”植株施肥。硅的应用显著增加了“女儿”植株的地上部鲜重。补充硅还增加了磷（P）、钾（K）和镁（Mg）的含量。此外，硅处理通过增加脯氨酸含量和抑制活性氧来减轻白粉病的损害。施用硅后，叶片表面菌丝的长度和密度降低。此外，覆盖有白粉病的“女儿”植物叶片的受感染面积减少。本研究还表明，硅增加了草莓的抗性基因的表达，降低了易感性基因的表达。总的来说，无论应用方法如何，硅的应用都促进了“女儿”植株的生长。建议在切割繁殖前直接向“女儿”植株叶面喷施硅，以提高其对白粉病的抗性。
