Department of Endocrinology, Institute of Zoology and Biomedical Research, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Krakow, Poland.
Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland.
Int J Mol Sci. 2022 Apr 2;23(7):3972. doi: 10.3390/ijms23073972.
The role of vitamin D has been confirmed in female reproductive organs. This study aimed to examine vitamin D metabolic enzymes, i.e., CYP27B1 and CYP24A1, mRNA transcript and protein abundance, and protein localization in the uterus of pigs on days 2-5, 10-12, 15-16 and 18-20 of the estrous cycle. Additionally, we determined 1,25(OH)D concentration in uterine flushings and the effect of 1,25(OH)D (10, 50 and 100 ng/mL) in vitro on and mRNA transcript abundance in endometrial and myometrial slices. In the endometrium, a greater mRNA transcript abundance was noted on days 10-12 and 18-20 than on days 15-16, whereas encoded protein abundance was greater on days 18-20 when compared to days 15-16. Endometrial mRNA transcript abundance was greater on days 18-20 than on days 10-12 and 15-16. In the myometrium, mRNA transcript abundance was greater on days 18-20 than on days 2-5 and 15-16, while protein abundance was larger in slices collected on days 18-20 than on days 15-16. Neither mRNA transcript nor encoded protein abundance were detected in the myometrium. The highest 1,25(OH)D concentration in uterine flushings was observed on days 18-20. Furthermore, the 1,25(OH)D increased the abundance of the mRNA transcript in endometrial slices. Overall, our results suggest that porcine uterus is an extra-renal site of vitamin D metabolism. Both the endometrium and the myometrium possess the ability to synthesize vitamin D, while only the endometrium contributes to its catabolism.
维生素 D 在女性生殖器官中的作用已得到证实。本研究旨在检测发情周期第 2-5 天、第 10-12 天、第 15-16 天和第 18-20 天母猪子宫中维生素 D 代谢酶，即 CYP27B1 和 CYP24A1 的 mRNA 转录本和蛋白丰度，以及蛋白定位。此外，我们还测定了子宫冲洗液中的 1,25(OH)D 浓度，并检测了 1,25(OH)D（10、50 和 100ng/mL）在体外对子宫内膜和子宫肌切片中 和 mRNA 转录本丰度的影响。在子宫内膜中，第 10-12 天和第 18-20 天的 mRNA 转录本丰度高于第 15-16 天，而蛋白丰度则在第 18-20 天高于第 15-16 天。子宫内膜 mRNA 转录本丰度在第 18-20 天高于第 10-12 天和第 15-16 天。在子宫肌层中，第 18-20 天的 mRNA 转录本丰度高于第 2-5 天和第 15-16 天，而蛋白丰度在第 18-20 天的切片中高于第 15-16 天。在子宫肌层中均未检测到 mRNA 转录本和编码蛋白的丰度。子宫冲洗液中 1,25(OH)D 的最高浓度出现在第 18-20 天。此外，1,25(OH)D 增加了子宫内膜切片中 mRNA 转录本的丰度。总的来说，我们的结果表明，猪的子宫是维生素 D 代谢的肾脏外部位。子宫内膜和子宫肌都有合成维生素 D 的能力，而只有子宫内膜参与其分解代谢。
