Graduate School of Health Science and Technology, Kawasaki University of Medical Welfare, Okayama, Japan.
Department of Clinical Nutrition, Faculty of Health Science and Technology, Kawasaki University of Medical Welfare, Okayama, Japan.
Biosci Biotechnol Biochem. 2022 Jun 25;86(7):884-894. doi: 10.1093/bbb/zbac054.
Caenorhabditis elegans contains two pathways for propionate metabolism, the vitamin B12-dependent and shunt pathways, which are similar to those in humans. In this study, we monitored the changes in propionate metabolism in the whole body of C. elegans using novel 13CO2 gas analysis. We measured the increase in 13CO2 excreted in the air after administering [1-13C]-labeled propionate ([13C]propionate) to the worms. The 13CO2 generated from [13C]propionate in C. elegans increased in a dose-dependent manner, reaching a maximum at 48 h. Enhanced expression of propionate metabolism-related genes was observed after administration of propionate. Knockdown of mmcm-1, which encodes the rate-limiting enzyme of the propionate metabolism, using RNAi reduced 13CO2 excretion. Thus, 13CO2 gas analysis could be confirmed 13CO2 excretion associated with changes in the metabolism of [13C]propionate in nematodes. This analysis can contribute to further understanding of the physiological effects of short-chain fatty acids.
秀丽隐杆线虫含有两条丙酸盐代谢途径，即维生素 B12 依赖途径和分流途径，这与人类的途径相似。在这项研究中，我们使用新型 13CO2 气体分析监测秀丽隐杆线虫整个机体中丙酸盐代谢的变化。我们测量了给予线虫[1-13C]标记的丙酸盐([13C]丙酸盐)后空气中排出的 13CO2 的增加量。[13C]丙酸盐在秀丽隐杆线虫中产生的 13CO2 呈剂量依赖性增加，在 48 小时达到最大值。给予丙酸盐后，观察到丙酸盐代谢相关基因的表达增强。使用 RNAi 敲低编码丙酸盐代谢限速酶的 mmcm-1 基因，可减少 13CO2 的排泄。因此，13CO2 气体分析可以证实与线虫中[13C]丙酸盐代谢变化相关的 13CO2 排泄。该分析有助于进一步了解短链脂肪酸的生理作用。
