Yuan Lin, Fu Qiang, Zhou Maojuan, Ma Yunqian, Zang Lihua, Qin Yingjian, Ji Dandan, Zhang Fengshan
College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Science) Jinan Shandong P. R. China 250353
Huatai Group Guangrao Shandong P. R. China 257335
RSC Adv. 2021 Feb 1;11(10):5506-5511. doi: 10.1039/d0ra10285g. eCollection 2021 Jan 28.
Polychlorinated biphenyls (PCBs) are synthetic organic compounds that are extremely difficult to break down in water and can accumulate in human fat and organisms. However, methods that can be used to detect large amounts of PCBs remain unsatisfactory, as they are generally overly sensitive and involve complex operations. An aptamer-based catalytic hairpin assembly (aptamer-CHA) reaction for the selective detection of 3,3',4,4'-tetrachlorobiphenyl (PCB 77) was developed. It combines the advantages of aptamers and signal amplification reactions. The aptamer selectivity recognizes the target, PCB 77, which triggers the sensitive CHA reaction to produce a fluorescence signal. CHA is a sensitive enzyme-free signal amplification method suitable for on-site detection. Therefore, the identification aptamer is the basis for the quantitative detection of PCB 77, with a detection range of 0.01 μg L to 500 μg L and a detection limit of 0.01 μg L. In this study, the aptamer was used to improve the selectivity of the reaction, and the CHA reaction improved the sensitivity of the detection system. Such high-sensitivity PCB detection capabilities with simplified procedures may be useful for real-time field detection and other monitoring tasks. This method can be used as a rapid fluorescence detection strategy for other targets in aquatic environments.
多氯联苯（PCBs）是合成有机化合物，极难在水中分解，且会在人体脂肪和生物体内蓄积。然而，可用于检测大量多氯联苯的方法仍不尽人意，因为这些方法通常过于灵敏且操作复杂。开发了一种基于适体的催化发夹组装（适体-CHA）反应，用于选择性检测3,3',4,4'-四氯联苯（PCB 77）。它结合了适体和信号放大反应的优点。适体选择性识别目标物PCB 77，触发灵敏的CHA反应产生荧光信号。CHA是一种适用于现场检测的灵敏的无酶信号放大方法。因此，识别适体是定量检测PCB 77的基础，检测范围为0.01 μg/L至500 μg/L，检测限为0.01 μg/L。在本研究中，适体用于提高反应的选择性，CHA反应提高了检测系统的灵敏度。这种具有简化程序的高灵敏度多氯联苯检测能力可能对实时现场检测和其他监测任务有用。该方法可作为水环境中其他目标物的快速荧光检测策略。
