Key Laboratory of Control of Quality and Safety for Aquatic Products, Minisrty of Agriculture, Chinese Academy of Fishery Sciences, Beijing, 100141, China.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Sci Rep. 2017 Jul 12;7(1):5191. doi: 10.1038/s41598-017-05352-7.
Gold nanoparticles (AuNP) dotted reduction graphene oxide (RGO-AuNP) is used as a platform for an aptamer biosensor to selectively detect 3,3'4,4'-polychlorinated biphenyls (PCB77). By anchoring aptamers onto the binding sites of RGO-AuNP and making use of the synergy effect of RGO and AuNP, the RGO-AuNP based biosensor exhibits superior analytical performances to AuNP based biosensor in terms of sensitivity and repeatability. The sensitivity of RGO-AuNP based aptamers (RGO-AuNP-Ap) biosensor (226.8 μA cm) is nearly two times higher than that of Au based biosensors (AuNP-Ap/Au electrode, 147.2 μA cm). The RGO-AuNP-Ap/Au biosensor demonstrated a linear response for PCB77 concentrations between 1 pg L and 10 μg L, with a low limit of detection (LOD) of 0.1 pg L. The superb LOD satisfies the exposure thresholds (uncontaminated water < 0.1 ng L) set out by International Agency for Research on Cancer (IARC) and the Environmental Protection Agency (EPA). The proposed biosensor can be a powerful tool for rapid, sensitive and selective detection of PCBs on site.
金纳米粒子（AuNP）点缀还原氧化石墨烯（RGO-AuNP）被用作适体生物传感器的平台，用于选择性地检测 3,3'4,4'-多氯联苯（PCB77）。通过将适体锚定在 RGO-AuNP 的结合位点上，并利用 RGO 和 AuNP 的协同效应，基于 RGO-AuNP 的生物传感器在灵敏度和重复性方面表现出优于基于 AuNP 的生物传感器的分析性能。基于 RGO-AuNP 的适体（RGO-AuNP-Ap）生物传感器的灵敏度（226.8 μA cm）几乎是基于 Au 的生物传感器（AuNP-Ap/Au 电极，147.2 μA cm）的两倍。RGO-AuNP-Ap/Au 生物传感器对 PCB77 浓度在 1 pg L 至 10 μg L 之间呈现线性响应，检测限（LOD）低至 0.1 pg L。卓越的 LOD 满足了国际癌症研究机构（IARC）和美国环境保护署（EPA）规定的暴露阈值（无污染水 < 0.1 ng L）。该生物传感器可作为现场快速、灵敏和选择性检测 PCBs 的有力工具。
