Campanella L, Tomassetti M, Bruni M R, Mascini M, Palleschi G
Food Addit Contam. 1986 Oct-Dec;3(4):277-88. doi: 10.1080/02652038609373594.
A new enzymatic-amperometric method for the determination of lecithin as an additive in foods and as a component of commercial drugs is proposed. The method is based on a detector realized with two enzymes, choline oxidase and phospholipase D, the former immobilized, the latter free in solution, and by an oxygen Clark electrode. The experimental conditions were investigated in order to obtain wide applications with different samples. Extraction or dissolution of the samples in ethanol proved satisfactory. The precision of the method was found to be about 1.5%, inaccuracy less than or equal to 4%. Correlation between the proposed enzymatic-amperometric method and an enzymatic-spectrophotometric reference method was satisfactory.
提出了一种新的酶促安培法，用于测定食品添加剂和商业药物成分中的卵磷脂。该方法基于一种由两种酶（胆碱氧化酶和磷脂酶D）构成的检测器，前者固定化，后者游离于溶液中，并结合氧克拉克电极。研究了实验条件，以便在不同样品中得到广泛应用。结果表明，样品在乙醇中的萃取或溶解效果良好。该方法的精密度约为1.5%，误差小于或等于4%。所提出的酶促安培法与酶促分光光度参考法之间的相关性良好。
