Chin C, Marks A, Yeger H, Kahn H, Baumal R
Hybridoma. 1986 Winter;5(4):339-46. doi: 10.1089/hyb.1986.5.339.
Monoclonal antibodies (MAbs) against a human sarcoma xenograft carried in BALB/c nu/nu mice were produced by immunizing BALB/c mice with tumor cells and fusing their spleens with the SP2/O-Ag 14 mouse myeloma cell line. Hybridoma supernatants were screened using cryostat tissue sections and an immunoperoxidase reaction for ability to stain osteosarcoma xenograft tumor cells but not tonsil lymphocytes. Of 73 supernatants tested, 19 reacted with both osteosarcoma tumor cells and lymphocytes, while three reacted only with osteosarcoma. One of the latter hybridomas was cloned by limiting dilution to establish a line producing an IgG1 MAb (OS-1). By immunoperoxidase, this MAb stained tumor cells in surgical biopsies of primary (6 of 7) and metastatic (1) osteosarcomas and showed limited cross-reactivity with other tumors. It also cross-reacted with some basement membranes, endothelium and muscular media of blood vessels, and smooth muscle, but not with parenchymal cells of various normal tissues. This MAb may prove useful for the immunohistochemical confirmation of a diagnosis of osteosarcoma in surgical pathology.
通过用肿瘤细胞免疫BALB/c小鼠并将其脾脏与SP2/O-Ag 14小鼠骨髓瘤细胞系融合，制备了针对携带于BALB/c nu/nu小鼠体内的人肉瘤异种移植物的单克隆抗体（MAb）。使用低温恒温器组织切片和免疫过氧化物酶反应筛选杂交瘤上清液，以检测其对骨肉瘤异种移植物肿瘤细胞而非扁桃体淋巴细胞的染色能力。在测试的73种上清液中，19种与骨肉瘤肿瘤细胞和淋巴细胞均发生反应，而3种仅与骨肉瘤发生反应。后者中的一种杂交瘤通过有限稀释法进行克隆，以建立产生IgG1 MAb（OS-1）的细胞系。通过免疫过氧化物酶法，该MAb可对原发性（7例中的6例）和转移性（1例）骨肉瘤手术活检中的肿瘤细胞进行染色，并显示与其他肿瘤的交叉反应有限。它还与一些基底膜、血管内皮和肌层以及平滑肌发生交叉反应，但不与各种正常组织的实质细胞发生反应。这种MAb可能在手术病理学中对骨肉瘤诊断的免疫组织化学确认有用。
