Department of Chemistry, BITS-Pilani Hyderabad Campus Shameerpet, Hyderabad, 500078, Telangana, India.
Chembiochem. 2022 Jul 5;23(13):e202200044. doi: 10.1002/cbic.202200044. Epub 2022 May 12.
Easily synthesizable, carbazole-based organic nanoaggregates have been designed for selective detection of D-(-)-ribose at physiological pH. The addition of D-ribose results in a ratiometric change in fluorescence color from green to cyan (LOD: ∼12 μM). The mechanistic studies indicate the presence of multipoint noncovalent interactions, such as hydrogen bonding and CH⋅⋅⋅π interactions between D-ribose and acyl hydrazone and terminal pyridyl units of the probe molecule. However, such multipoint interactions dissociate the preformed self-assembled nanoclusters and induce change in optical response. The probe molecule was further exploited in analyzing D-ribose content in biological fluids (diluted human urine and blood serum) and oral supplements. The small standard deviation values (2-3.8 %) with nearly quantitative recovery (93.5-105.5 %) indicate the high accuracy of the presented method. Further, low-cost portable device based coated paper strips were designed for 'on-location' rapid, detection of D-ribose even at remote locations.
已设计出易于合成的基于咔唑的有机纳滤体，可在生理 pH 值下选择性检测 D-(-)-核糖。加入 D-核糖会导致荧光颜色从绿色到青色的比率变化（LOD：约 12 μM）。机理研究表明，存在多点非共价相互作用，例如 D-核糖与酰腙和探针分子末端吡啶单元之间的氢键和 CH⋅⋅⋅π 相互作用。然而，这种多点相互作用会使预先形成的自组装纳米簇解离，并引起光学响应的变化。该探针分子进一步用于分析生物流体（稀释的人尿和血清）和口服补充剂中的 D-核糖含量。小的标准偏差值（2-3.8%）和接近定量的回收率（93.5-105.5%）表明该方法具有很高的准确性。此外，还设计了基于廉价便携式涂层纸条的低成本便携式设备，即使在偏远地区也可进行“现场”快速检测 D-核糖。
