Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; Shanghai Ocean University, College of Fisheries and Life Science, Shanghai 201106, China.
Reprod Biol. 2022 Jun;22(2):100640. doi: 10.1016/j.repbio.2022.100640. Epub 2022 Apr 13.
Forkhead box L2 (FOXL2) is a forkhead transcription factor essential for proper reproductive function in females and plays a crucial role in ovarian development in many species of vertebrates. However, little research on goose FOXL2 gene has been conducted. In this study, the cDNA and genomic DNA sequences of goose FOXL2 gene were cloned and characterized. The goose FOXL2 is a single exon gene and contains one open reading frame of 918 bp encoding a protein of 305 amino acids. Bioinformatics analysis displays that the deduced FOXL2 amino acid sequence contains the highly conserved forkhead box domain, which shares greatest similarity to avian species, especially to that of ducks and chicken. RT-qPCR analysis indicates that the FOXL2 mRNA is widely expressed in all examined tissues of fertilized female eggs (28 days), and differentially expressed in female adult (70 days) and laying Zhedong White geese (270 days). Meanwhile, FOXL2 is highly expressed in the hypothalamic-pituitary-ovarian axis, especially in the ovary tissues of the adult and laying geese. Furthermore, one microsatellite (TGTC1415-1418----) and five single nucleotide polymorphisms (A1290G, G1495A, T1554C, T1692A, C1695G and T1697G) were identified in the 3'-untranslated regions. All the information derived from this study could be valuable and facilitate further researches on the function of FOXL2 gene in geese.
叉头框 L2（FOXL2）是一种叉头转录因子，对雌性生殖功能至关重要，在许多脊椎动物物种的卵巢发育中起着关键作用。然而，对鹅 FOXL2 基因的研究较少。本研究克隆和鉴定了鹅 FOXL2 基因的 cDNA 和基因组 DNA 序列。鹅 FOXL2 是一个单外显子基因，包含一个 918bp 的开放阅读框，编码一个 305 个氨基酸的蛋白质。生物信息学分析显示，推导的 FOXL2 氨基酸序列含有高度保守的叉头框结构域，与禽类，尤其是鸭和鸡的 FOXL2 具有最大的相似性。RT-qPCR 分析表明，FOXL2 mRNA 在受精雌鹅（28 天）所有检测组织中广泛表达，并在雌鹅（70 天）和产蛋浙东白鹅（270 天）中差异表达。同时，FOXL2 在下丘脑-垂体-卵巢轴中高度表达，尤其是在成年和产蛋鹅的卵巢组织中。此外，在 3'-非翻译区鉴定到一个微卫星（TGTC1415-1418----）和五个单核苷酸多态性（A1290G、G1495A、T1554C、T1692A、C1695G 和 T1697G）。本研究获得的所有信息都可能具有重要价值，并有助于进一步研究 FOXL2 基因在鹅中的功能。
