Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF, Campos dos Goytacazes, RJ, Brazil.
NUPEN, Universidade Federal do Rio de Janeiro (UFRJ), Macaé, RJ, Brazil.
Pestic Biochem Physiol. 2022 May;183:105082. doi: 10.1016/j.pestbp.2022.105082. Epub 2022 Mar 17.
Schinus terebinthifolius, Raddi, has been extensively studied due to its anti-inflammatory and antibiotic properties. S. terebinthifolius was also toxic to some insects, however little has been explored about the nature of its insecticide compounds or the toxicity of this plant to insect species. In this work, we investigate the toxicity of S. terebinthifolius seed flour against the insect C. maculatus. S. terebinthifolius seed flour interfered with the post hatch development of the C. maculatus larvae, decreasing larval survival, mass and length. Using DEAE-cellulose chromatography, five protein fractions were isolated, a non-retained fraction (NRF) and four retained fractions, eluted with 0.25, 0.5, 0.7 and 1.0 M NaCl. Proteins with varying molecular masses were observed in all fractions. The majority protein bands were identified by mass spectrometry analysis and among the main identified proteins are 11S globulins (such glycinin), lipoxygenase, chitinases, 7S globulins (vicilins, canavalin and β conglycinin), annexin, catalase and sucrose binding protein. All DEAE-protein fractions were toxic to the insect, interfering with the post hatch larval development and survival. Decreases greater than 90% were observed in the larval mass and length at 20 days after oviposition (DAO) for larvae raised on diet containing 0.5% of some fractions. Alterations in the level of proteins, glucose and in the activity of the enzymes lipases and cysteine proteases were also detected in these larvae. Our results show that seeds of S. terebinthifolius have an arsenal of toxic proteins with potential for the control of the insect C. maculatus.
巴西番樱桃因具有抗炎和抗生素特性而受到广泛研究。然而，巴西番樱桃对一些昆虫也具有毒性，但关于其杀虫剂化合物的性质或该植物对昆虫物种的毒性，人们知之甚少。在这项工作中，我们研究了巴西番樱桃种子粉对昆虫 C. maculatus 的毒性。巴西番樱桃种子粉干扰了 C. maculatus 幼虫的孵化后发育，降低了幼虫的存活率、质量和长度。使用 DEAE-纤维素层析法，分离出五个蛋白质馏分，一个非保留馏分（NRF）和四个保留馏分，用 0.25、0.5、0.7 和 1.0 M NaCl 洗脱。所有馏分中都观察到具有不同分子量的蛋白质。通过质谱分析鉴定了大多数蛋白带，主要鉴定的蛋白包括 11S 球蛋白（如glycinin）、脂氧合酶、几丁质酶、7S 球蛋白（vicilins、canavalin 和 β conglycinin）、膜联蛋白、过氧化氢酶和蔗糖结合蛋白。所有 DEAE-蛋白馏分对昆虫都有毒性，干扰孵化后幼虫的发育和存活。在产卵后 20 天（DAO），用含有 0.5%某些馏分的饮食饲养的幼虫的幼虫质量和长度下降超过 90%。在这些幼虫中还检测到蛋白质、葡萄糖水平以及脂肪酶和半胱氨酸蛋白酶活性的改变。我们的结果表明，巴西番樱桃的种子具有潜在的控制昆虫 C. maculatus 的毒性蛋白武器库。
