Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF, Campos dos Goytacazes, RJ 28013-602, Brazil.
Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF, Campos dos Goytacazes, RJ 28013-602, Brazil.
Pestic Biochem Physiol. 2024 Mar;200:105829. doi: 10.1016/j.pestbp.2024.105829. Epub 2024 Feb 13.
Cowpea weevil, Callosobruchus maculatus, is the primary pest of stored cowpea seeds. The management of this infestation currently relies on insecticides, resulting in environmental pollution and selection of insecticide-resistant pests. Consequently, research efforts are being devoted to identify natural insecticides as sustainable and environment friendly alternatives for the control of C. maculatus. In this study, we explore the toxic effects of the nonhost seeds Parkia multijuga, Copaifera langsdorffii, Ormosia arborea, Amburana cearensis, Lonchocarpus guilleminianus, Sapindus saponaria, and Myroxylon peruiferum, on the cowpea weevil C. maculatus. Notably, all nonhost seeds led to reductions between 60 and 100% in oviposition by C. maculatus females. Additionally, the larvae were unable to penetrate the nonhost seeds. Artificial seeds containing 0.05% to 10% of cotyledon flour were toxic to C. maculatus larvae. Approximately 40% of larvae that consumed seeds containing 0.05% of O. arborea failed to develop, in contrast to control larvae. Proteomic analysis of A. cearensis and O. arborea seeds identify revealed a total of 371 proteins. From those, 237 are present in both seeds, 91 were exclusive to O. arborea seeds, and 43 were specific to A. cearensis seeds. Some of these proteins are related to defense, such as proteins containing the cupin domain and 11S seed storage protein. The in silico docking of cupin domain-containing proteins and 11S storage protein with N-acetylglucosamine (NAG) showed negative values of affinity energy, indicating spontaneous binding. These results showed that nonhost seeds have natural insecticide compounds with potential to control C. maculatus infestation.
豇豆象，Callosobruchus maculatus，是储存豇豆种子的主要害虫。目前，这种虫害的管理依赖于杀虫剂，导致环境污染和杀虫剂抗性害虫的选择。因此，研究人员正在努力寻找天然杀虫剂作为控制 C. maculatus 的可持续和环保替代品。在这项研究中，我们探讨了 Parkia multijuga、Copaifera langsdorffii、Ormosia arborea、Amburana cearensis、Lonchocarpus guilleminianus、Sapindus saponaria 和 Myroxylon peruiferum 等非宿主种子对豇豆象 C. maculatus 的毒性影响。值得注意的是，所有非宿主种子都导致 C. maculatus 雌虫产卵减少 60%至 100%。此外，幼虫无法穿透非宿主种子。含有 0.05%至 10%子叶粉的人工种子对 C. maculatus 幼虫有毒。与对照幼虫相比，约有 40%食用含 0.05% O. arborea 种子的幼虫未能发育。A. cearensis 和 O. arborea 种子的蛋白质组分析共鉴定出 371 种蛋白质。其中，237 种存在于两种种子中，91 种仅存在于 O. arborea 种子中，43 种仅存在于 A. cearensis 种子中。其中一些蛋白质与防御有关，如含有 cupin 结构域和 11S 种子贮藏蛋白的蛋白质。Cupin 结构域蛋白和 11S 贮藏蛋白与 N-乙酰葡萄糖胺（NAG）的计算机对接显示亲和力能量的负值，表明自发结合。这些结果表明，非宿主种子具有控制 C. maculatus 侵染的天然杀虫剂化合物。
