Study on Lung Injury Caused by Fine Particulate Matter and Intervention Effect of .

OBJECTIVE

The objective of this study was to observe the protective effect of drops in a rat model of fine particulate matter (PM2.5) lung injury.

METHODS

Forty male Wistar rats were randomly divided into blank control (NC), normal saline (NS), PM2.5-infected (PM), and (RW) groups. Rats in the NC group were not provided any interventions, whereas those in the NS and PM groups were administered normal saline and PM2.5 suspension by trachea drip once a week for four weeks. Rats in the RW group were intraperitoneally administered for 14 days and then administered PM2.5 suspension by trachea drip 7 days after drug delivery. The levels of inflammatory factors such as interleukin-6, interleukin-1, and tumor necrosis factor-alpha and oxidative stress biomarkers such as 8-hydroxy-2'-deoxyguanosine, 4-hydroxynonenal, and protein carbonyl content were determined in the serum and bronchoalveolar lavage fluid by ELISA. The level of 4-hydroxynonenal in the lung was also determined using Western blotting and immunohistochemical staining.

RESULTS

Levels of inflammatory factors and oxidative stress biomarkers were all increased in the PM group but decreased in the RW group. Western blotting revealed increased 4-hydroxynonenal levels in the PM group but decreased levels in the RW group. Immunohistochemical staining also provided similar results.

CONCLUSION

could protect rats from inflammation and oxidative stress injury caused by PM2.5.