Collier D S, Pain J A, Wight D G, Lovat P, Bailey M E
J Pathol. 1986 Nov;150(3):187-94. doi: 10.1002/path.1711500307.
Kupffer cell phagocytic function is reduced in the presence of obstructive jaundice. To investigate possible mechanisms we report a study of the rat liver in extrahepatic cholestasis, using light microscopy, immunohistochemistry and electron microscopy, Immunohistochemistry was performed with monoclonal antibodies specific for rat Kupffer cells ED 1, ED 2 and ED 3 and monoclonal antibodies directed against class II antigens of the rat major histocompatibility complex Ox 3 and Ox 6. Extrahepatic cholestasis was produced by bile duct ligation. In bile duct ligated animals light microscopy showed proliferation of bile ductules and an increase in sinusoidal cells. Immunohistochemistry with ED 1, ED 2 and ED 3 demonstrated a marked increase in the number of positive cells, but few of these cells were positive with Ox 3 and Ox 6, whereas the proliferating bile ductules were strongly positive. Electron microscopy revealed two homogeneous granular substances within the sinusoidal lumen and loss of the space of Disse. Despite a reduction in Kupffer cell phagocytic function in obstructive jaundice there is an increase in Kupffer cells, but these cells appear to be in an inactivated state as few express class II antigens on their surface. Furthermore the granular substance within the space of Disse may interfere with function.
在存在梗阻性黄疸的情况下，库普弗细胞的吞噬功能会降低。为了探究可能的机制，我们报告了一项对肝外胆汁淤积大鼠肝脏的研究，采用了光学显微镜、免疫组织化学和电子显微镜技术。使用针对大鼠库普弗细胞ED 1、ED 2和ED 3的单克隆抗体以及针对大鼠主要组织相容性复合体II类抗原Ox 3和Ox 6的单克隆抗体进行免疫组织化学检测。通过胆管结扎造成肝外胆汁淤积。在胆管结扎的动物中，光学显微镜显示胆小管增生和窦状隙细胞增多。用ED 1、ED 2和ED 3进行免疫组织化学检测显示阳性细胞数量显著增加，但这些细胞中很少有对Ox 3和Ox 6呈阳性反应的，而增生的胆小管则呈强阳性。电子显微镜显示窦状隙腔内有两种均匀的颗粒物质以及狄氏间隙消失。尽管在梗阻性黄疸中库普弗细胞吞噬功能降低，但库普弗细胞数量增加，不过这些细胞似乎处于失活状态，因为很少有细胞在其表面表达II类抗原。此外，狄氏间隙内的颗粒物质可能会干扰功能。
