Carpino F, Gaudio E, Marinozzi G, Melis M, Motta P M
J Submicrosc Cytol. 1981 Oct;13(4):581-98.
The rat liver after extrahepatic biliary obstruction was studied by SEM and TEM in correlation with basic histochemical techniques. Cholestasis was verified by serological methods. The biochemical data (increase in serum bilirubin values, a gradual lowering of the albumin fraction), in agreement with the ultrastructural results of a sparse RER, suggested a gradual decrease of the protein synthetic activity of the hepatocyte. SEM and TEM revealed numerous fat-storing cells, closely associated with patches of connective fibrils in the subendothelial spaces. Further ultrastructural observations demonstrated: a) a proliferation of the intrahepatic biliary tree (ductular proliferation, including newly formed ducts with sacculation and diverticuli); b) an increased number of canaliculo-ductular junctions and, c) an increase in the length of the bile canalicular network due to its tortuous course, pocketing and side branching. The occurrence of an intact cytoplasmic barrier separating the bile canalicular lumen from the Disse's space together with the results obtained by retrograde infusion of ferritin into the biliary tree suggested that the regurgitation pathway by ductular reabsorption and by transhepatocytic transport is the best documented and most acceptable, at least in the rat.
通过扫描电子显微镜（SEM）和透射电子显微镜（TEM），并结合基本组织化学技术，对肝外胆管阻塞后的大鼠肝脏进行了研究。通过血清学方法证实了胆汁淤积。生化数据（血清胆红素值升高，白蛋白部分逐渐降低）与粗面内质网稀疏的超微结构结果一致，提示肝细胞的蛋白质合成活性逐渐降低。扫描电子显微镜和透射电子显微镜显示出大量贮脂细胞，它们与内皮下间隙中的结缔组织纤维束紧密相连。进一步的超微结构观察表明：a）肝内胆管树增生（小胆管增生，包括新形成的具有囊状扩张和憩室的胆管）；b）胆小管-小胆管连接数量增加；c）胆小管网络因走行迂曲、成袋状和侧支分支而长度增加。将铁蛋白逆行注入胆管树所获得的结果，以及存在将胆小管腔与狄氏间隙分隔开的完整细胞质屏障，提示至少在大鼠中，通过小胆管重吸收和经肝细胞转运的反流途径是有充分证据且最可接受的。
