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数字显微摄影中的开源智能手机适配器

Open-source Smartphone Adapter in Digital Photomicrography.

作者信息

Mondal Himel, Mondal Shaikat

机构信息

Department of Physiology, Fakir Mohan Medical College and Hospital, Balasore, Odisha, India.

Department of Physiology, Raiganj Government Medical College and Hospital, Raiganj, West Bengal, India.

出版信息

J Microsc Ultrastruct. 2022 Feb 15;10(1):7-9. doi: 10.4103/JMAU.JMAU_45_20. eCollection 2022 Jan-Mar.

Abstract

BACKGROUND AND AIM

Smartphones can be used to capture images from the microscope. There are commercial and homemade adapters that can be used to overcome the difficulty of focusing on a smartphone camera. We conducted this study to test if the usage of a homemade adapter reduces the time and effort of the operator in comparison to the free-hand technique in smartphone photomicrography.

MATERIALS AND METHODS

We made a simple smartphone adapter for digital photomicrography. Thirty-two operators first captured the image on the smartphone with the free-hand technique and then with the adapter thrice from a microscope. The time for focusing was compared statistically by paired -test. A survey was conducted to know operators's opinions on adapter-assisted photomicrography.

RESULT

All the participants were able to capture images from the microscopes. The average time for focusing with adapter was (11.89 ± 4.19 seconds) significantly ( = 0.0001) lower than the free-hand technique (25.56±11.81 seconds). However, the images yielded from both techniques were of equal quality. The majority of the participant found the method to be easy and reduce their effort in focusing and capturing an image.

CONCLUSION

Low-cost homemade smartphone adapter helps in reducing the time required for capturing an image from the microscope. It decreases the effort of the operator in comparison to the free-hand technique. Hence, it may be a good choice for those who need to capture images from the microscopes frequently for either telemedicine or research purpose in resource-limited settings.

摘要

背景与目的

智能手机可用于从显微镜获取图像。有商业和自制的适配器可用于克服智能手机摄像头聚焦困难的问题。我们开展本研究以测试与智能手机显微摄影中的徒手技术相比,使用自制适配器是否能减少操作者的时间和精力。

材料与方法

我们制作了一个用于数码显微摄影的简易智能手机适配器。32名操作者首先用徒手技术在智能手机上拍摄图像,然后使用适配器从显微镜拍摄三次。通过配对检验对聚焦时间进行统计学比较。进行了一项调查以了解操作者对适配器辅助显微摄影的看法。

结果

所有参与者都能从显微镜获取图像。使用适配器聚焦的平均时间为(11.89±4.19秒),显著低于徒手技术(25.56±11.81秒)(P = 0.0001)。然而,两种技术获得的图像质量相当。大多数参与者认为该方法简单,减少了他们聚焦和拍摄图像的精力。

结论

低成本的自制智能手机适配器有助于减少从显微镜获取图像所需的时间。与徒手技术相比,它减少了操作者的精力。因此,对于那些在资源有限的环境中出于远程医疗或研究目的需要频繁从显微镜获取图像的人来说,它可能是一个不错的选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2d/9012408/a862188bd7db/JMAU-10-7-g001.jpg

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