Wilson M E, Mainprize T H, Friesen P D, Miller L K
J Virol. 1987 Mar;61(3):661-6. doi: 10.1128/JVI.61.3.661-666.1987.
The nucleotide sequence of the cDNA of an abundant late 0.5-kilobase transcript of Autographa californica nuclear polyhedrosis virus revealed a small open reading frame encoding an arginine-rich 6.9-kilodalton protein. The predicted amino acid composition of the 6.9-kilodalton protein was essentially identical to that of the core protein of viral nucleocapsids. The precise location of the 5' and 3' ends of the transcript were confirmed by S1 nuclease and primer extension analyses. Multiple overlapping transcripts through this region include three early and three abundant late RNAs which are transcribed counterclockwise and one transient RNA which is transcribed clockwise with respect to the physical map of the virus.
苜蓿银纹夜蛾核型多角体病毒一种丰富的晚期0.5千碱基转录本的cDNA核苷酸序列，揭示了一个小的开放阅读框，其编码一种富含精氨酸的6.9千道尔顿蛋白。预测的6.9千道尔顿蛋白的氨基酸组成与病毒核衣壳核心蛋白的氨基酸组成基本相同。通过S1核酸酶和引物延伸分析确定了转录本5′和3′末端的精确位置。通过该区域的多个重叠转录本包括三个早期和三个丰富的晚期RNA，它们相对于病毒物理图谱逆时针转录，以及一个瞬时RNA，其相对于病毒物理图谱顺时针转录。
