Yong Xin, Mao Lejiao, Seaman Matthew N J, Jia Da
Key Laboratory of Birth Defects and Related Diseases of Women and Children, Department of Paediatrics, West China Second University Hospital, State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, China.
Cambridge Institute for Medical Research, University of Cambridge, The Keith Peters Building, Cambridge Biomedical Campus, CB2 0XY, UK.
iScience. 2022 May 20;25(5):104254. doi: 10.1016/j.isci.2022.104254. Epub 2022 Apr 13.
Complex mechanisms govern the sorting of membrane (cargo) proteins at endosomes to ensure that protein localization to the post-Golgi endomembrane system is accurately maintained. Endosomal retrieval complexes mediate sorting by recognizing specific motifs and signals in the cytoplasmic domains of cargo proteins transiting through endosomes. In this review, the recent progress in understanding the molecular mechanisms of how the retromer complex, in conjunction with sorting nexin (SNX) proteins, operates in cargo recognition and sorting is discussed. New data revealing the importance of different SNX proteins and detailing how post-translational modifications can modulate cargo sorting to respond to changes in the environment are highlighted along with the key role that endosomal protein sorting plays in SARS-CoV-2 infection.
复杂的机制控制着内体中膜（货物）蛋白的分选，以确保蛋白在高尔基体后内膜系统中的定位得以精确维持。内体回收复合体通过识别穿过内体的货物蛋白胞质结构域中的特定基序和信号来介导分选。在本综述中，我们讨论了在理解逆转录复合物与分选连接蛋白（SNX）协同作用于货物识别和分选的分子机制方面的最新进展。同时强调了新数据，这些数据揭示了不同SNX蛋白的重要性，并详细说明了翻译后修饰如何调节货物分选以应对环境变化，以及内体蛋白分选在SARS-CoV-2感染中所起的关键作用。
