Institute of Plant Biology, College of Life Science, Zhejiang University, Hangzhou 310058, China.
Yi Chuan. 2022 Apr 20;44(4):313-321. doi: 10.16288/j.yczz.21-415.
Flanking genomic sequences refer to the DNA sequences flanking specific sites of known sequences in chromosome, which contain information such as candidate genes, transcriptional regulation, chromosome structure, and biosafety, and play an important role in genomics research. Flanking sequence acquisition technologies are mainly used in the cloning of regulatory sequences such as promoters and enhancers, identification of T-DNA or transposon insertion sites, chromosome walking, genome-wide gap filling, etc. It is an important means of structural genomics research and functional genomics research. It is applied in the identification of transgenic plants and animals and their safety management. With the development of molecular biology, many methods for obtaining flanking sequences have been established, including plasmid rescue, inverse PCR, ligation-mediated PCR, semi-random primer PCR, whole-genome resequencing etc. In this review, we summarize and compared different methods for acquiring flanking genomic sequence. The principles and research progress of each approach are discussed.
侧翼基因组序列是指染色体上已知序列特定位点两侧的 DNA 序列，包含候选基因、转录调控、染色体结构和生物安全性等信息，在基因组学研究中发挥着重要作用。侧翼序列获取技术主要用于启动子和增强子等调控序列的克隆、T-DNA 或转座子插入位点的鉴定、染色体步移、全基因组缺口填充等。它是结构基因组学研究和功能基因组学研究的重要手段。它应用于转基因动植物的鉴定及其安全性管理。随着分子生物学的发展，已经建立了许多获取侧翼序列的方法，包括质粒拯救、反向 PCR、连接介导的 PCR、半随机引物 PCR、全基因组重测序等。本文对不同的侧翼基因组序列获取方法进行了总结和比较，讨论了每种方法的原理和研究进展。
