Hermán F, Hadházy P, Magyar K
Thromb Res. 1986 Dec 1;44(5):575-85. doi: 10.1016/0049-3848(86)90160-x.
The disaggregating effect of PGI2 was measured in a modified in vivo model described by Hornstra. The arterial blood of mongrel dogs was directed through an extracorporeal filter by a roller pump, and the pressure proximal to the filter was measured. The filter became spontaneously occluded mostly by aggregates of platelets within a few minutes, and as a result the pressure before the filter increased continuously. PGI2, administered either before the filter or intravenously, produced dose-related reduction in filtration pressure. Results obtained in control experiments revealed that the rate of platelet aggregation was reproducible; neither the count of platelets in the circulating blood nor the in vitro sensitivity of platelets to ADP and PGI2 changes significantly in the course of an experiment. The technique described seems to be useful to determine the disaggregatory potency of prostacyclin and other substances.
在霍恩斯特拉描述的一种改良体内模型中测定了前列环素（PGI2）的解聚作用。杂种狗的动脉血由滚压泵引导通过体外过滤器，并测量过滤器近端的压力。在几分钟内，过滤器大多因血小板聚集而自发阻塞，结果过滤器前的压力持续升高。在过滤器前给药或静脉注射PGI2，均可使过滤压力呈剂量相关降低。对照实验的结果表明，血小板聚集率是可重复的；在实验过程中，循环血液中的血小板计数以及血小板对二磷酸腺苷（ADP）和PGI2的体外敏感性均无明显变化。所描述的技术似乎有助于确定前列环素和其他物质的解聚效力。
