Nakamura K, Imada Y, Maeda M
Vet Pathol. 1986 Nov;23(6):712-7. doi: 10.1177/030098588602300610.
Specific-pathogen-free 10-week-old chickens were inoculated via the air sac with Escherichia coli and showed lymphocytic depletion of bursa of Fabricius and thymus. In experiment I, chickens were necropsied at 12 and 24 hours, 2, 3, and 5 days after inoculation. At 12 hours after inoculation there was lymphocytic depletion in the medulla of lymphoid follicles of the bursa. At 24 hours after inoculation there was lymphocytic depletion also in the cortex of follicles and edema in interfollicular interstitium and follicular medulla. At 2 and 3 days after inoculation there were more marked lymphocytic depletion in medulla and cortex, and fibrosis in interfollicular interstitium. Partial repopulation of follicles with lymphocytes was seen at 5 days after inoculation. In the thymus, lymphocytic depletion occurred in the cortex. At 12 hours after inoculation, lymphocytic necrosis increased in number more than that of control chickens. The width of the cortex and medulla decreased. At 24 hours after inoculation, lymphocytic necrosis increased further. At 2 to 5 days after inoculation, the boundary between the cortex and medulla of lobules was obscure and cellular elements of the cortex and medulla were mingled. In experiment II, chickens were necropsied as in experiment I and also at 8 and 14 days after inoculation. The relative weights of the bursa and thymus reduced rapidly to minimal relative weights at 8 days after inoculation. At 14 days after inoculation, both bursa and thymus had normal relative weights and histological structures. These findings indicate that E. coli infection may induce transient lymphocytic depletion of lymphoid tissues in the chicken.
将10周龄的无特定病原体鸡通过气囊接种大肠杆菌后，法氏囊和胸腺出现淋巴细胞耗竭。在实验I中，接种后12小时、24小时、2天、3天和5天对鸡进行剖检。接种后12小时，法氏囊淋巴滤泡髓质出现淋巴细胞耗竭。接种后24小时，滤泡皮质也出现淋巴细胞耗竭，滤泡间间质和滤泡髓质出现水肿。接种后2天和3天，髓质和皮质的淋巴细胞耗竭更明显，滤泡间间质出现纤维化。接种后5天可见滤泡有部分淋巴细胞重新填充。在胸腺中，皮质出现淋巴细胞耗竭。接种后12小时，淋巴细胞坏死数量比对照鸡增加。皮质和髓质宽度减小。接种后24小时，淋巴细胞坏死进一步增加。接种后2至5天，小叶皮质和髓质之间的边界模糊，皮质和髓质的细胞成分混合。在实验II中，鸡的剖检时间与实验I相同，此外还在接种后8天和14天进行剖检。接种后8天，法氏囊和胸腺的相对重量迅速降至最低相对重量。接种后14天，法氏囊和胸腺的相对重量及组织结构均正常。这些发现表明，大肠杆菌感染可能诱导鸡淋巴组织的短暂淋巴细胞耗竭。
