Zhang Huanhuan, Phillip Fesobi Olumide, Wu Linnan, Zhao Fengyun, Yu Songlin, Yu Kun
The Key Laboratory of Characteristics of Fruit and Vegetable Cultivation and Utilization of Germplasm Resources of the Xinjiang Production and Construction Corps, Shihezi University, Xinjiang, China.
Front Plant Sci. 2022 Apr 4;13:859395. doi: 10.3389/fpls.2022.859395. eCollection 2022.
Malus sieversii grows on the slopes of the Tianshan Mountains in Xinjiang where the difference in daily temperature is significant. In recent years, the rhizosphere soil health of has been severely impacted by anthropogenic disturbance and pathogenic infestation. The soil nutrient content and soil microorganism diversity are the main components of soil health. Low temperature has negative effects on soil bacterial community structure by inhibiting the accumulation of carbon and nitrogen. However, the effects of temperature and nitrogen application on soil carbon and nitrogen accumulation and the bacterial community composition in the rhizosphere soil of are unclear. We set two temperature levels, i.e., low temperature (L) and room temperature (R), combined with no nitrogen (N) and nitrogen application (N) to explore the response of plant carbon and nitrogen uptake, rhizosphere soil carbon and nitrogen accumulation and bacterial community composition to temperature and nitrogen fertilization. At the same temperature level, plant C abundance (P-AtomC), plant N absolute abundance (P-ConN), soil N abundance (S-AtomN) and soil urease, protease and glutaminase activities were significantly higher under nitrogen application compared with the no-nitrogen application treatment. The bacterial community diversity and richness indices of the apple rhizosphere soil in the N treatment were higher than those in the N treatment. The relative abundances of , , and were higher in the LN treatment than in the LN treatment. Redundancy analysis (RDA) showed that plant C absolute abundance (P-ConC) and plant N absolute abundance (P-ConN) were the main factors affecting the soil bacterial community composition. In summary, Nitrogen application can alleviate the effects of low temperature stress on the soil bacterial community and is of benefit for the uptakes of carbon and nitrogen in plants.
新疆野苹果生长在新疆天山山脉的山坡上，这里昼夜温差显著。近年来，其根际土壤健康受到人为干扰和病原菌侵染的严重影响。土壤养分含量和土壤微生物多样性是土壤健康的主要组成部分。低温通过抑制碳氮积累对土壤细菌群落结构产生负面影响。然而，温度和施氮对新疆野苹果根际土壤碳氮积累及细菌群落组成的影响尚不清楚。我们设置了两个温度水平，即低温（L）和室温（R），并结合不施氮（N）和施氮（N）处理，以探究植物碳氮吸收、根际土壤碳氮积累及细菌群落组成对温度和施氮的响应。在相同温度水平下，与不施氮处理相比，施氮处理下植物碳丰度（P-AtomC）、植物氮绝对丰度（P-ConN）、土壤氮丰度（S-AtomN）以及土壤脲酶、蛋白酶和谷氨酰胺酶活性均显著更高。N处理下苹果根际土壤的细菌群落多样性和丰富度指数高于N处理。LN处理中、、和的相对丰度高于LN处理。冗余分析（RDA）表明，植物碳绝对丰度（P-ConC）和植物氮绝对丰度（P-ConN）是影响土壤细菌群落组成的主要因素。综上所述，施氮可缓解低温胁迫对土壤细菌群落的影响，有利于新疆野苹果植株对碳和氮的吸收。
