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长链非编码 RNA SBF2-AS1 通过靶向 miR-520a-3p 促进非小细胞肺癌进展。

LncRNA SBF2-AS1 Facilitates Nonsmall Cell Lung Cancer Progression by Targeting miR-520a-3p.

机构信息

Department of Respiration, The People's Hospital of Zhangqiu Area, Jinan 250200, China.

Occupational Disease Department, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250001, China.

出版信息

J Healthc Eng. 2022 Apr 11;2022:2223149. doi: 10.1155/2022/2223149. eCollection 2022.

DOI:10.1155/2022/2223149
PMID:35444785
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9015877/
Abstract

BACKGROUND

Long noncoding RNA (lncRNA) SET-binding factor 2 (SBF2) antisense RNA1 (SBF2-AS1), which acts as an oncogene in various cancers, can promote tumors progression. The study aimed to explore the role and molecular mechanism of SBF2-AS1 in nonsmall cell lung cancer (NSCLC).

METHODS

qRT-PCR was introduced to detect SBF2-AS1 and miR-520a-3p expression in NSCLC. The effects of SBF2-AS1 and miR-520a-3p on the proliferation, migration, and invasion of NSCLC cells were assessed through cell counting kit-8 (CCK-8) and transwell assay. Furthermore, the relationship of SBF2-AS1 and miR-520a-3p was verified by the RNA immunoprecipitation (RIP) assay, dual-luciferase assay, and Spearman correlation analysis.

RESULTS

In NSCLC tissues, SBF2-AS1 was highly expressed, while miR-520a-3p expression has decreased. The overall survival of NSCLC patients with high SBF2-AS1 expression was lower. SBF2-AS1 silencing repressed the proliferation, migration, and invasion of NSCLC cells. SBF2-AS1 directly interacted with miR-520a-3p, and a negative relationship was observed between their expression levels in NSCLC tissues. More importantly, the suppression of SBF2-AS1 silencing on the proliferation, migration, and invasion in NSCLC cells was counteracted by miR-520a-3p inhibition.

CONCLUSION

SBF2-AS1 accelerated the proliferation, migration, and invasion of NSCLC cells via mediating miR-520a-3p, thus promoting NSCLC progression.

摘要

背景

长链非编码 RNA（lncRNA）SET 结合因子 2（SBF2）反义 RNA1（SBF2-AS1）作为多种癌症中的致癌基因，能够促进肿瘤进展。本研究旨在探讨 SBF2-AS1 在非小细胞肺癌（NSCLC）中的作用及分子机制。

方法

采用 qRT-PCR 检测 NSCLC 中 SBF2-AS1 和 miR-520a-3p 的表达。通过细胞计数试剂盒-8（CCK-8）和 Transwell 实验评估 SBF2-AS1 和 miR-520a-3p 对 NSCLC 细胞增殖、迁移和侵袭的影响。进一步通过 RNA 免疫沉淀（RIP）实验、双荧光素酶报告基因实验和 Spearman 相关性分析验证 SBF2-AS1 和 miR-520a-3p 之间的关系。

结果

在 NSCLC 组织中，SBF2-AS1 高表达，而 miR-520a-3p 表达降低。SBF2-AS1 高表达的 NSCLC 患者总生存率较低。沉默 SBF2-AS1 抑制 NSCLC 细胞的增殖、迁移和侵袭。SBF2-AS1 可与 miR-520a-3p 直接结合，且二者在 NSCLC 组织中的表达呈负相关。更为重要的是，miR-520a-3p 抑制剂可拮抗 SBF2-AS1 沉默对 NSCLC 细胞增殖、迁移和侵袭的抑制作用。

结论

SBF2-AS1 通过介导 miR-520a-3p 加速 NSCLC 细胞的增殖、迁移和侵袭，从而促进 NSCLC 进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/19eac2ac208b/JHE2022-2223149.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/60656c3a0804/JHE2022-2223149.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/fc9519d85f7c/JHE2022-2223149.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/abd31b08ee13/JHE2022-2223149.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/5b847d513c89/JHE2022-2223149.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/19eac2ac208b/JHE2022-2223149.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/60656c3a0804/JHE2022-2223149.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/fc9519d85f7c/JHE2022-2223149.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/abd31b08ee13/JHE2022-2223149.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/5b847d513c89/JHE2022-2223149.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a06a/9015877/19eac2ac208b/JHE2022-2223149.005.jpg

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