Gheorghe Constantinescu Elena Codruţa, Ionescu Mihaela, Pirici Daniel, Birceanu Adelina, Stroescu Cezar, Copcă Narcis, Şurlin Valeriu, Burtea Daniela Elena, Săftoiu Adrian
PhD student, Research Center of Gastroenterology and Hepatology, University of Medicine and Pharmacy of Craiova, Romania.
Department of Medical Informatics, University of Medicine and Pharmacy of Craiova, Romania.
Curr Health Sci J. 2021 Oct-Dec;47(4):529-538. doi: 10.12865/CHSJ.47.04.08. Epub 2021 Dec 31.
Hepatic steatosis has been identified as an independent risk factor for post-operative complications. The aim of our research was to assess how inflammation and neoangiogenesis associated with different stages of hepatic steatosis are related to post-operative complications in patients who undergo hepatic resection.
Our study included 19 patients with hepatic steatosis undergoing liver resection for primary or secondary tumors. For every patient we performed immunostaining using a panel of 5 primary antibodies (CD3, CD20, CD68, CD31, CD34) to highlight inflammation and neoangiogensis in the non-tumoral hepatic parenchyma.
Taking into consideration the number of vessels as well as the signal area and integrated optical density (IOD) forCD3, CD20, CD68, and also the degree of steatosis, the univariate analysis with a log-rank (Mantel-Cox) test revealed that patients with higher values of CD31 and CD34 had a higher rate of post-operative complications on a 30-day follow-up period. Also, we used a Mann-Whitney U and Kruskal-Wallis H tests for group distributions. We noticed thatCD34 was significantly increased in patients diagnosed with steatosis compared to the control group and there was a statistically significant difference between CD31 median values of S0 (27.6) and S1 (55.8) grades.
Patients with steatosis that presented higher values of CD31 and CD34 had a higher rate of post-operative complications. Further studies should assess the value of pre-operative evaluation of angiogenesis in patients with liver steatosis submitted to liver surgery.
肝脂肪变性已被确定为术后并发症的独立危险因素。我们研究的目的是评估与不同阶段肝脂肪变性相关的炎症和新生血管形成如何与接受肝切除手术患者的术后并发症相关。
我们的研究纳入了19例因原发性或继发性肿瘤接受肝切除的肝脂肪变性患者。对于每位患者，我们使用一组5种一抗（CD3、CD20、CD68、CD31、CD34）进行免疫染色，以突出非肿瘤性肝实质中的炎症和新生血管形成。
考虑到血管数量以及CD3、CD20、CD68的信号面积和积分光密度（IOD），以及脂肪变性程度，采用对数秩（Mantel-Cox）检验进行单因素分析显示，在30天随访期内，CD31和CD34值较高的患者术后并发症发生率较高。此外，我们使用Mann-Whitney U检验和Kruskal-Wallis H检验进行组间分布分析。我们注意到，与对照组相比，诊断为脂肪变性的患者CD34显著增加，S0级（27.6）和S1级（55.8）的CD31中位数之间存在统计学显著差异。
CD31和CD34值较高的脂肪变性患者术后并发症发生率较高。进一步的研究应评估对接受肝脏手术的肝脂肪变性患者进行术前血管生成评估的价值。
