Damiano R E, Van Horn D L, Schultz R O
Ann Ophthalmol. 1978 Apr;10(4):479-85.
Corneal buttons were cut from cat and human donor eyes by anterior trephination (with or without scissor completion) and from excised corneoscleral rims by posterior trephination (with or without a press). Scanning electron microscopy was used to evaluate the slope and quality of the cut edge, the diameter of the section of Descemet's membrane that was actually cut, and the loss of endothelial cells around the cut edge. With anterior trephination, the diameter of the section of Descemet's membrane that is cut was found to be less than that of the trephine because the cut edge slopes inward. Posterior trephination with or without a press consistently produced circular buttons with accurately cut outward-sloping edges. This resulted in a diameter of Descemet's membrane which was greater than in the anteriorly trephinated buttons. There also was less damage to peripheral Descemet's membrane and endothelium with the posterior method, especially when compared to scissor cut buttons. A 360 degree peripheral ring of Descemet's membrnae (0.05 to 0.28 mm in width) was denuded of endothelial cells with all techniques.
通过前板层角膜环切术（有或没有剪刀辅助完成）从猫和人类供体眼中获取角膜纽扣，通过后板层角膜环切术（有或没有压模）从切除的角巩膜缘获取角膜纽扣。使用扫描电子显微镜评估切割边缘的斜率和质量、实际切割的Descemet膜切片的直径以及切割边缘周围内皮细胞的损失。在前板层角膜环切术中，发现切割的Descemet膜切片直径小于环钻直径，因为切割边缘向内倾斜。有或没有压模的后板层角膜环切术始终产生边缘向外倾斜且切割精确的圆形纽扣。这导致Descemet膜的直径大于前板层角膜环切术获得的纽扣。与剪刀切割的纽扣相比，后一种方法对周边Descemet膜和内皮的损伤也更小。所有技术都会使Descemet膜的360度周边环（宽度为0.05至0.28毫米）的内皮细胞剥脱。
