Fortibui Maxine Mambo, Jang Mina, Lee Sohyun, Ryoo In-Ja, Ahn Jong Seog, Ko Sung-Kyun, Kim Jinheung
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.
ACS Appl Bio Mater. 2022 May 16;5(5):2232-2239. doi: 10.1021/acsabm.2c00084. Epub 2022 Apr 21.
Acetylcholinesterase (AChE) is a pivotal enzyme that is closely related with multiple neurological diseases, such as brain disorders or alterations in the neurotransmission and cancer. The development of convenient methods for imaging AChE activity in biological samples is very important to understand its mechanisms and functions in a living system. Herein, a fluorescent probe exhibiting emission in the near-infrared (NIR) region is developed to detect AChE and visualize biological AChE activities. This probe exhibits a quick response time, reasonable detection limit, and a large Stokes shift accompanied by the NIR emission. The probe has much better reactivity toward AChE than butyrylcholinesterase, which is one of the significant interfering substances. The outstanding specificity of the probe is proved by cellular imaging AChE activity and successful mapping in different regions of zebrafish. Such an effective probe can greatly contribute to ongoing efforts to design emission probes that have distinct properties to assay AChE in biological systems.
乙酰胆碱酯酶（AChE）是一种关键酶，与多种神经系统疾病密切相关，如脑部疾病或神经传递改变以及癌症。开发用于成像生物样品中AChE活性的便捷方法对于理解其在活体系统中的机制和功能非常重要。在此，开发了一种在近红外（NIR）区域发射荧光的探针来检测AChE并可视化生物AChE活性。该探针具有快速的响应时间、合理的检测限以及伴随着近红外发射的大斯托克斯位移。该探针对AChE的反应性比丁酰胆碱酯酶好得多，丁酰胆碱酯酶是一种重要的干扰物质。通过细胞成像AChE活性和在斑马鱼不同区域的成功映射证明了该探针具有出色的特异性。这样一种有效的探针可以极大地推动正在进行的设计具有独特性质以在生物系统中测定AChE的发射探针的努力。
