29853Vision Research Foundation, Chennai, Tamil Nadu, India.
Medical Research Foundation, 29853Sankara Nethralaya, Chennai, Tamil Nadu, India.
J Biomater Appl. 2022 Sep;37(3):375-388. doi: 10.1177/08853282221091042. Epub 2022 Apr 21.
The signalling response is determined by the cell's reaction to different biochemical and biophysical inputs such as stiffness, topological, and structural alignment. The surface patterns at the nano-scale can be an influential factor in cell signalling behaviour. It is important to understand the cellular response to the biophysical cues for biomedical applications. Biomaterials have an important role in regenerative tissue engineering. In this study, we have fabricated electrospun polycaprolactone (PCL) and PCL- (PCL-AV) nanofibrous matrix and studied its effect on the human tenon fibroblast (HTF) cellular and morphological changes. The electrospun fibers were characterized using Scanning Electron Microscope (SEM), Fourier Transform Infrared spectroscopy (FTIR), Atomic Force Microscopy (AFM) and Brunaur, Emette and Teller BET) analysis for their morphology, composition, topography, surface area and porosity. The results revealed fiber size, roughness and porosity has been altered by addition of AV. The HTF cell viability, proliferation and expression of focal adhesion proteins, such as FAK, Ezrin, Vasp and Cofilin on the PCL-AV fiber matrix were examined. The results showed a change in cellular morphology and a significant change in the cofilin phosphorylation on PCL-AV nanofiber. The influence of composition on the nano-dimension of the PCL has made a significant impact on the cellular morphology at both gene and protein levels. This observation suggests that AV composition in the nanofiber can significantly influence the HTF cellular adhesions.
信号反应取决于细胞对不同生化和生物物理输入的反应，如硬度、拓扑和结构排列。纳米尺度的表面图案可能是细胞信号转导行为的一个影响因素。了解细胞对生物物理线索的反应对于生物医学应用非常重要。生物材料在再生组织工程中起着重要的作用。在这项研究中，我们制备了聚己内酯（PCL）和 PCL-（PCL-AV）纳米纤维基质，并研究了其对人腱成纤维细胞（HTF）细胞和形态变化的影响。使用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、原子力显微镜（AFM）和 Brunauer、Emette 和 Teller BET）分析对静电纺丝纤维进行了形态、组成、形貌、比表面积和孔隙率的表征。结果表明，添加 AV 后，纤维的尺寸、粗糙度和孔隙率发生了变化。研究了 PCL-AV 纤维基质上 HTF 细胞活力、增殖以及粘着斑蛋白（如 FAK、Ezrin、Vasp 和 Cofilin）的表达。结果表明，PCL-AV 纳米纤维上细胞形态发生了变化，Cofilin 磷酸化明显发生了变化。组成对 PCL 纳米尺寸的影响对细胞形态在基因和蛋白质水平上都有显著影响。这一观察结果表明，纳米纤维中的 AV 组成可以显著影响 HTF 细胞的黏附。
