Department of Food Science and Nutrition, College of Food and Agricultural Science, King Saud University, Riyadh, Saudi Arabia.
PLoS One. 2022 Apr 21;17(4):e0267499. doi: 10.1371/journal.pone.0267499. eCollection 2022.
Flowering plants are valuable in numerous ways, including food/feed supply for living organisms, fuel production, and medicinal uses. Several plant extracts/products are used to treat variety of serious ailments in human and animals. Lavandula angustifolia is a flowering plant that possesses anti-inflammatory and anti-depressive medicinal properties. Cancer is a deadly disorder affecting millions of people globally. It affects several human organs, including liver, stomach, and lungs. Several researchers are doing efforts to eliminate the disease around the globe. In this study, Chang and Huh-7 liver cell lines were utilized as human normal hepatocyte model and innovation to mimic the liver environment. Cytotoxicity of L. angustifolia seed extracts was investigated at two different concentrations (50% and 100%) against Chang and Huh-7 liver cell lines by colorimetric assay which is used to assess cell metabolic activities. The Chang and Huh-7 liver cell lines were treated with L. angustifolia seeds extracts (50% and 100%) and incubated for 24 and 48 hours under standard conditions (37°C, 5% CO2). The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay was employed to quantify cell survival. Seed extracts of L. angustifolia exerted varied cytotoxic effects depending on the concentration and treatment duration. The results indicated that L. angustifolia seed extracts with 100% concentration exhibited the highest cytotoxicity against Huh-7 and Chang liver cell lines. In conclusion, L. angustifolia seed extracts exhibited cytotoxic activity which can be enhanced based on the concentration and treatment duration. The findings of the current study are critical for the development of novel herbal-based therapies for fatal disorders such as liver cancer. However, more investigations are required to reveal cytotoxicity mechanisms of L. angustifolia seed extracts.
开花植物在许多方面都很有价值，包括为生物提供食物/饲料供应、燃料生产和药用用途。一些植物提取物/产品被用于治疗人类和动物的各种严重疾病。薰衣草具有抗炎和抗抑郁的药用特性。癌症是一种影响全球数百万人的致命疾病。它影响包括肝脏、胃和肺在内的多个人类器官。许多研究人员正在努力在全球范围内消除这种疾病。在这项研究中，Chang 和 Huh-7 肝细胞系被用作人类正常肝细胞模型和创新模型，以模拟肝脏环境。通过比色法研究了薰衣草种子提取物在两个不同浓度（50%和 100%）下对 Chang 和 Huh-7 肝细胞系的细胞毒性，比色法用于评估细胞代谢活性。将 Chang 和 Huh-7 肝细胞系用薰衣草种子提取物（50%和 100%）处理，并在标准条件（37°C，5%CO2）下孵育 24 和 48 小时。使用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物测定法来量化细胞存活率。薰衣草种子提取物根据浓度和处理时间的不同，表现出不同的细胞毒性作用。结果表明，薰衣草种子提取物浓度为 100%时，对 Huh-7 和 Chang 肝细胞系的细胞毒性最强。总之，薰衣草种子提取物表现出细胞毒性活性，这种活性可以根据浓度和处理时间的增加而增强。本研究的结果对于开发基于草药的新型治疗方法治疗肝癌等致命疾病至关重要。然而，还需要进一步的研究来揭示薰衣草种子提取物的细胞毒性机制。
