Department of Health Inspection, College of Public Health, Shenyang Medical College, Shenyang, P. R. China.
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P. R. China.
J Sep Sci. 2022 Jul;45(13):2310-2320. doi: 10.1002/jssc.202200054. Epub 2022 Apr 27.
In this study, the hydroxypropyl β-cyclodextrin hybrid monolithic material was fabricated and firstly applied as an adsorbent for dispersive solid-phase extraction coupled with high-performance liquid chromatography to detect trace-level seven fluoroquinolones in water samples. The prepared hydroxypropyl β-cyclodextrin hybrid monolithic material was characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, and adsorption experiments, which showed excellent specific adsorption to the target fluoroquinolones. Under the optimized conditions, the extraction methodology showed satisfactory precision with relative standard deviations between 2.6% and 5.6%, good linearity (R ≥ 0.9990), and satisfactory recoveries (82.5-91.8%). The limits of detection and limits of quantification of the method were in the range of 0.4-1.2 and 1.4-4.0 ng/mL, respectively, which confirmed the possibility of quantifying trace levels. Furthermore, the material could be reused at least five times. These results demonstrated that the hydroxypropyl β-cyclodextrin hybrid monolithic material was a promising adsorbent for fluoroquinolones, and the established method combined dispersive solid-phase extraction with high-performance liquid chromatography was suitable for the determination of fluoroquinolones in aqueous samples.
在本研究中，制备了羟丙基-β-环糊精杂化整体材料，并首次将其作为一种吸附剂，用于高效液相色谱与分散固相萃取联用，检测水样中的痕量七种氟喹诺酮类药物。通过傅里叶变换红外光谱、扫描电子显微镜和吸附实验对制备的羟丙基-β-环糊精杂化整体材料进行了表征，结果表明该材料对目标氟喹诺酮类药物具有优异的特异性吸附能力。在优化条件下，该萃取方法具有良好的精密度，相对标准偏差在 2.6%至 5.6%之间，线性良好（R ≥ 0.9990），回收率令人满意（82.5%至 91.8%）。该方法的检出限和定量限分别在 0.4-1.2 和 1.4-4.0 ng/mL 范围内，证实了定量痕量水平的可能性。此外，该材料至少可重复使用五次。这些结果表明，羟丙基-β-环糊精杂化整体材料是一种很有前途的氟喹诺酮类药物吸附剂，建立的高效液相色谱与分散固相萃取联用方法适用于水样中氟喹诺酮类药物的测定。
