Environmental filtering dominated the antibiotic resistome assembly in river networks.

River networks play important roles in dissemination of antibiotic resistance genes (ARGs). The occurrence, diversity, and abundance of ARGs in river networks have been widely investigated. However, the assembly processes that shaped ARGs profiles across space and time are largely unknown. Here, the dynamics of ARGs profiles in river networks (Taihu Basin) were revealed by high-throughput quantitative PCR followed by multiple statistical analyses to assess the underlying ecological processes. The results revealed clear variations for ARGs profiles across wet, normal, and dry seasons. Meanwhile, a significant negative correlation (p < 0.01) was observed between the similarity of ARGs profiles and geographic distance, indicating ARGs profiles exhibited distance-decay patterns. Null model analysis showed that ARGs profiles were mainly assembled via deterministic processes. Redundancy analysis followed by hierarchical partitioning revealed that environmental attributes (mainly pH and temperature) were the major factors affecting the dynamics of ARGs profiles. Together, these results indicated that environmental filtering was the dominant ecological process that shaped ARGs profiles. This study enhances our understanding how the antibiotic resistome is assembled in river networks and will be beneficial for the development of management strategies to control ARGs dissemination.