College of Agronomy, Hunan Agricultural University, Changsha, China.
School of Minerals Processing and Bioengineering, Central South University, Changsha, China.
Chemosphere. 2022 Aug;301:134655. doi: 10.1016/j.chemosphere.2022.134655. Epub 2022 Apr 18.
Most previous studies have focused on the diversity and species richness of microbial communities, however, understanding the interactions between species and detecting key functional members of the community can help us better understand how microorganisms perform their functions. In this study, the response of the rice plant microbial community to the inoculation of cadmium-resistant endophytic bacterium R5 (Stenotrophomonas) was investigated for the first time using a microbial phylogenetic molecular ecological network. The results showed that inoculation of R5 changed the topological characteristics of the microbial network in rice plants, with the resulting network displaying stronger complexity and interaction in roots and aboveground parts, indicating that inoculation of R5 provided favorable conditions for microbial interactions. In addition, these interactions may be related to the absorption and transportation of cadmium by rice. Under the exogenous addition of R5, the network interactions of the rice plant microbial community were more inclined to cooperation. Both in the roots and aboveground parts of rice, the plant Cd content showed a decrease as the complexity and connectivity of the network increased, suggesting that complex microbial networks may be more beneficial to rice than simple microbial networks because as they were more adaptive and resistant to unfavorable environments. After inoculation with the R5 strain, the negative interaction with Cd content in rice plants increased significantly, and there might be more synergy between the microbial community and plants to jointly inhibit the absorption and transportation of Cd.
大多数先前的研究都集中在微生物群落的多样性和物种丰富度上，然而，了解物种之间的相互作用以及检测群落中的关键功能成员可以帮助我们更好地理解微生物如何发挥其功能。在这项研究中，首次使用微生物系统发育分子生态网络研究了水稻植物微生物群落对耐镉内生细菌 R5（寡养单胞菌）接种的反应。结果表明，R5 的接种改变了水稻植物微生物网络的拓扑特征，导致根系和地上部分的网络显示出更强的复杂性和相互作用，这表明 R5 的接种为微生物相互作用提供了有利条件。此外，这些相互作用可能与水稻对镉的吸收和运输有关。在外源添加 R5 的情况下，水稻植物微生物群落的网络相互作用更倾向于合作。在水稻的根和地上部分，随着网络复杂性和连通性的增加，植物 Cd 含量呈下降趋势，这表明复杂的微生物网络可能比简单的微生物网络更有利于水稻，因为它们更具适应性和对不利环境的抵抗力。接种 R5 菌株后，水稻植物中与 Cd 含量的负相互作用显著增加，微生物群落与植物之间可能存在更多的协同作用，共同抑制 Cd 的吸收和运输。
