通过液体厌氧消化，驯化稻田微生物对稻草进行多次水解以生产甲烷。
Multiple hydrolyses of rice straw by domesticated paddy soil microbes for methane production via liquid anaerobic digestion.
机构信息
State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China.
State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China.
出版信息
Bioresour Technol. 2022 Jun;354:127184. doi: 10.1016/j.biortech.2022.127184. Epub 2022 Apr 18.
The aim of this study was to investigate the hydrolysis of rice straw (RS) using domesticated paddy soil microbes (DPSMs) with swine wastewater (SW) as the nitrogen source and the multiple hydrolyses for CH production via liquid anaerobic digestion (L-AD). Three hydrolyses of RS with a 45% inoculation ratio (IR) under the conditions of a carbon/nitrogen ratio (C/N ratio) of 40, temperature of 37 °C, inoculum/substrate ratio (I/S ratio) of 2:1, and immersion depth of 6.0 cm were optimal, attaining maximum volatile fatty acids (VFAs) after five days, possibly owing to the synergistic effect of aerobic microbes (Firmicutes and Actinomycetes) and anaerobic microbes (Bacteroidetes and Acidobacteria). After three hydrolyses, the degradation rates of hemicellulose, cellulose, and lignin in RS were 88.45%, 83.19% and 70.09%, respectively. The accumulative CH production reached 462.11 mL/g VS after three hydrolyses, and its curve fitted well with the modified Gompertz model (R > 0.984).
本研究旨在利用驯化的稻田微生物（DPSM）和猪废水（SW）作为氮源，通过液体厌氧消化（L-AD）进行多次水解来生产 CH。在 45%接种率（IR）、碳氮比（C/N 比）为 40、温度为 37°C、接种物/底物比（I/S 比）为 2:1 和浸没深度为 6.0cm 的条件下，对 RS 进行了三次水解，在五天后达到最大的挥发性脂肪酸（VFAs），这可能是由于好氧微生物（Firmicutes 和 Actinomycetes）和厌氧微生物（Bacteroidetes 和 Acidobacteria）的协同作用。三次水解后，RS 中半纤维素、纤维素和木质素的降解率分别为 88.45%、83.19%和 70.09%。三次水解后，累计 CH 产量达到 462.11 mL/g VS，其曲线与修正的 Gompertz 模型拟合良好（R>0.984）。
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