Marques Samuel Pedro Dantas, Owen Robert Wyn, da Silva Ana Maria Amaral, Alves Neto Manoel Lourenço, Trevisan Maria Teresa Salles
Instituto Federal de Educação, Ciência e Tecnologia do Ceará, Departamento de Química, Av. José de Freitas Queiroz, 5000, Quixadá, CE CEP: 63902-580, Brazil; Programa de Pós-graduação em Química, Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici - Bloco 935 superior - Laboratório de Produtos Naturais e Biotecnologia (LPNBio), CP: 60451-970 Fortaleza, CE, Brazil.
Programa de Pós-graduação em Química, Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici - Bloco 935 superior - Laboratório de Produtos Naturais e Biotecnologia (LPNBio), CP: 60451-970 Fortaleza, CE, Brazil.
Food Chem. 2022 Sep 15;388:132964. doi: 10.1016/j.foodchem.2022.132964. Epub 2022 Apr 12.
We hypothesised that QuEChERS could be successfully applied to the extraction of bitter acids and xanthohumol from hops, which would be less time consuming, cheaper, and more eco-friendly by the severe reduction of solvent use. High performance liquid chromatography was used to separate the compounds after extraction and quantitation was evaluated against standard calibration curves for bitter acids prepared from an International calibration extract (ICE-4) and an authentic standard of xanthohumol. The standard QuEChERS method was compared to mini and micro-versions including clean-up and spiking procedures. The quantitative analyzes indicate the applicability of the QuEChERS method for the quantitation of bitter acids compared to Soxhlet extraction. The statistical data confirm reproducibility of the total alpha- and beta- acids measured by the standard method and the modified mini- and micro-QuEChERS procedures. Our hypothesis is supported by the data described and is consistent with other previous methods described in the literature.
我们假设，快速、简便、廉价、高效的样品前处理方法（QuEChERS）能够成功应用于啤酒花中苦味酸和黄腐酚的提取，通过大幅减少溶剂使用，该方法将更省时、更便宜且更环保。萃取后，采用高效液相色谱法分离化合物，并根据由国际校准提取物（ICE-4）制备的苦味酸标准校准曲线和黄腐酚的真实标准品对定量进行评估。将标准QuEChERS方法与包括净化和加标程序的微型和超微型版本进行了比较。定量分析表明，与索氏提取法相比，QuEChERS方法适用于苦味酸的定量分析。统计数据证实了标准方法以及改良的微型和超微型QuEChERS程序所测得的总α-酸和β-酸的重现性。我们的假设得到了所述数据的支持，并且与文献中描述的其他先前方法一致。
